DNA expert Dr. Robin Cotton testified for nearly the entire day on RFLP analysis of blood evidence from four key crime scene items: Ron Goldman's boot, the Bundy walkway, Rockingham foyer, and a sock. Judge Ito ruled that the prosecution must present statistical frequency analysis when introducing DNA mixture evidence—a significant victory for the defense arguing that jury confusion otherwise results. The day centered on heated methodological disputes between the prosecution and defense regarding how to disaggregate and calculate probabilities for mixed DNA samples.

• Judge Ito ruled the prosecution must provide statistical frequency data for DNA mixture evidence or jury will be misled about odds of match
• Dr. Cotton testified that Goldman's boot stain (item 78) contained DNA mixture with Nicole Brown included and Goldman inconclusive/excluded; Simpson definitively excluded
• Bundy walkway stain (item 52) and Rockingham foyer stain (item 12) both showed Simpson matches across all five genetic markers tested; Brown and Goldman excluded
• Sock evidence (item 13) matched Nicole Brown at all five markers; Simpson and Goldman excluded
• Defense challenged Cotton's methodology of using band intensity to disaggregate mixtures, arguing NRC-recommended aggregation method should be used
• Foundation objections to DNA testimony largely overruled; judge left open possibility of motion to strike
• Motion hearing on EDTA discovery and prosecutor Goldberg's improper hypothetical questions; judge declined sanctions