First of all, Mr. Harmon, at the beginning of his direct examination, mentioned that there were three areas that you were reporting on today and that is results from the Bronco, results from the sock and results from the rear gate at Bundy; is that correct?
And as far as the results in the Bronco is concerned, sample 25 had already been tested by LAPD and was a DQ-Alpha of 1.1, 1.2; is that correct?
And the additional data that you presented to us, I guess, is on no. 26, which was also a drop on the floor mat?
Yes. I don't know the exact distribution as far as it being a drop or a smear, but it was some blood on the floor.
And no indication of an 18, 18 allele that would indicate a mixture with Nicole Brown Simpson?
Okay. All right. Now, let's move to the rear gate--to the sock, excuse me, and what you told us about is blood that was found, I guess, on the sock that was up on the calf area near the top of the sock?
All right. And basically what you've reported to us today is some additional probes that you conducted on that sample; is that correct?
Well, this--this was the first time I had presented any of the RFLP data on this sample.
But actually I think the last time you were here, just so we don't confuse things, even though you didn't present the data, we had a discussion, did we not, on the fact that you had been able to conduct RFLP testing and you had done some probes on that?
Okay. Now, so basically what you are here to tell us is that you did more probes there; is that right?
Okay. Now, could we turn to what is Defense 1192-B previously in evidence, a chart.
And the last time that we discussed--the time that you were here we had a discussion of the amount of DNA and the nature of the DNA on the rear gate. Do you recall that?
And as a matter of fact, on your--in your testimony for the Prosecution you had presented an analysis of the concentration of the DNA that was found on the three samples in the rear gate; 115, 116 and 117. Do you recall that?
And then we went over a comparison of the swatch DNA samples at 115, 116 and 117 based on your data. Do you recall that?
And the basis of this comparison was to take what are known as nanograms, which is sometimes shortened as NG; is that correct?
Yes. It was the nanograms of the DNA recovered to the milligrams of the swatch material that was sampled.
Right. And this was part of what you were doing to try to give us an assessment of concentration; is that correct?
All right. Could we show the comparison, because Mr. Harmon did ask you something here about why you didn't do 115, 116 and the amount of that. Now, in the comparison of the swatch DNA sample to 115 and 116, you found that there was two times as much DNA in those samples as there was to no. 6 which was a drop recovered at Rockingham?
15 times as much as 47, which is the first--which is a drop found at Bundy, correct?
Now, this comparison is in terms of the amount of DNA, but wouldn't it be fair to say that certainly as far as 47, 48, 49 and 50 are concerned that what you are finding there with respect to those blood drops is that there was bacterial degradation of those samples?
Yes. Again we discussed this in detail last time, but that is my interpretation as to what most likely happened is that there was a bacterial degradation of those samples.
Right. And as far as 115 and 116 are concerned, by the use of your yield gel and slot-blot analysis you did not find bacterial degradation in those samples?
I did not detect that same bacterial degradation type pattern that I saw in those other samples.
KEY QUOTENow, 1192-A is the comparison of DNA sample to 117 and that is the back gate sample for which you were able to do RFLP testing; is that correct?
And you found four times as much, the concentration here to be four times as much--Howard; is that correct?
All right. We found in terms of concentration four times as much as Rockingham drop?
Okay. Now, again with respect to 117, in terms of your slot-blot analysis and your yield gel analysis, you did not find the same evidence of bacterial degradation and contamination that you found on 47, 48, 49, 50 and 52?
Well, again I think we talked a little bit about this, but you recall there were only three of those five that you mentioned that we actually had a yield gel on where we could see the bacterial degradation pattern.
Now, finally, one other measure of whether or not there is bacterial contamination in samples, as reflected in RFLP testing, is your ability to detect, umm, DNA bands at the top of the RFLP membranes; is that correct?
Yes. The bands that are at the top of the membrane, the top portion, those would tend to be the higher--well, they are the higher molecular weight DNA bands and those are more subject to degradation than the lower bands.
And that the process of bacterial contamination is such that the bacteria begins to eat away and it is going to get the larger fragments earlier than the smaller fragments?
Well, it will--it will chop down the larger fragments so they tend to get smaller is what happens and they tend to degrade.
All right. And if you were to have significant or even substantial--I think we used those two terms--
-- to make a distinction in regard to bacterial degradation, but if you were to have either significant or substantial bacterial degradation, what you would tend to find is that you might either lose bands at a top of an RFLP gel or they would become faint in comparison to other bands?
Now, on these samples, for example, I think it is--you can refer to your notes--D1S7, is a sample where you found on 117 high molecular weight DNA band at the top of the gel at around 10,028 base pairs?
All right. And literally that means that there is--those, you know--well, tell the jury exactly what that means in terms of base pairs rather than have me describe it.
Yes. Well, I think we've had some discussion about base pairs in DNA and what we are talking about there is the g pairing with the c and the a pairing with the t and each one of those is a base pair. So this would be 10,300 of those is the size of the fragment, so if you could visualize this, you recall seeing these--the hydrogen bonds bringing the two DNA strands together, there would be 10,000 some odd of these units together and that is the size of this fragment after the restriction enzyme is cut into the pieces.
And just to illustrate the point, your Honor, may I remove from the light box and put up for a second on the elmo, just so everybody can see exactly which autorad I'm referring to--
All right. Mr. Scheck, are you going to use the rest of the items on the box, because the glare is--
D4S139, would you agree that that is another probing that had bands that appear to be in the upper range?
All right. Let the record reflect that I am just pointing for the jury to the particular autorad that has those--that I have just--Mr. Sims and I were just discussing.
And now I'm moving to another one that is entitled D5S110, and again, would that--that has some bands at the top of the gel?
All right. And as far as the D4S139 and the D5S110, those are pretty clear and distinct bands at the top of those gels?
And that is consistent with your finding of no significant or substantial bacterial contamination on sample 117?
And I would suppose D17S26, the upper band there is also in the upper regions of the gel?
Somewhat. Now we are getting a little more toward what I consider the middle range, but that is 68, 6900.
And could you just--if do you me a favor and come down here and just circle the high molecular weight band on 117. This is on the D1S7 autorad that I think you told us was something--something on the order of 10,030 base pairs; is that correct?
Yes. That is typically what is seen, because there is a lot more copies of where the probe is hitting in the higher band than in the lower band, so when you see a spread like that, where the bands are quite spread out across the--from the top and bottom into the gel, that usually the lower band is significantly weaker than the upper band.
But the fact that the upper band, which is--is more distinct and more intense than the lower band, that is another good indication that we don't have any significant or substantial bacterial contamination with 117?
270 times as much as 49?
I did not detect that same bacterial degradation type pattern that I saw in those other samples.
For the rear gate, 117, it is about 10,342, so 10,342 base pairs.
The fact that the upper band, which is more distinct and more intense than the lower band, that is another good indication that we don't have any significant or substantial bacterial contamination with 117?