Mr. Sims, we have a few additional results that were not available the last time you testified?
Okay. Let's just go in numerical order starting with the Bronco. Did your laboratory perform PCR testing on LAPD item no. 25, which was removed from the driver's side carpet of the Bronco?
Okay. What result was obtained from stain no. 25 on the driver's side carpet of the Bronco?
Could you step up to the board here, the Bronco exhibit or result board, rather, no. 260 and let's start with no. 25. I made some patches here. Did you assign a DOJ DNA number to LAPD item no. 25?
Okay. May the record reflect, your Honor, I'm going to put a patch in that blank column reflecting DOJ DNA 39.
Now, there has previously been testimony from Collin Yamauchi that he performed DQ-Alpha testing and produced the result 1.1, 1.2.
May the record reflect that I have another patch that reflects both the DQ-Alpha and the D1S80 results?
What I would like you to do, Mr. Sims, you've got that black marker, I would like you in the lower portion of the "Frequency" column, would you please write in the D1S80 only frequency for stain no. 25 or your no. 39?
Yes, just the lower--could you put D1S80 and then what the frequency range would be for the type 24, 25.
So the range is from one in 29 and what group would the one in 29 reflect the frequency of?
Your Honor, we have attached a patch. Could we move that down, Mr. Fairtlough, that reflects LAPD 26. May the record reflect we have just taped it onto the top of People's exhibit no. 260.
Mr. Sims--and that is your DOJ DNA no. 40, LAPD item 26 from the floor mat driver's side?
Could you write in the frequency for the D1S80 type for the result that you've obtained which is consistent with Mr. Simpson.
Okay. Could you we move on to the sock photo board and sock result board. The sock result board is 262 and the sock photo board is 262-A.
Now, Mr. Sims, what sort of testing have you performed on sock 42 or sock a which you've designated for stain 42A-3? What kind of test have you performed since you were here last?
The tests that were completed--and actually these were begun in February, but the RFLP process takes several weeks because of the low amount of DNA on this particular sample that was applied to our gel and we tested it for the restriction fragment length polymorphism results as distinguished from the PCR test that we talked about, such as the PCR tests again under DQ-Alpha and D1S80. These are the RFLP tests now that we performed.
Okay. And when you began those tests did you already know what the PCR results were on the various stains on those socks?
A-3 is--is near the top, toward the top portion of the sock designated A. You notice that it is on the same side as the cut-out that we did previously--the previous RFLP on, which was A-1 stain, so now this is the A-3 stain up near the top, (Indicating).
So A-1 is the stain that you produced RFLP results which show the consistency with Nicole Brown?
Okay. How many different probes--or strike that. What samples did you have during this RFLP run? What other samples did you use during the same run?
Yes. Because I already knew what the PCR tests were, I ran three fix samples on that particular RFLP gel. One was the sock 42A-3 stain, another was Mr. Simpson's reference sample for side-by-side comparison. And then finally I ran LAPD no. 117, which is our DNA no. 49 which was collected from the rear gate at the Bundy crime scene.
Okay. How many different probes were you able to submit 42A-3 to when you conducted the RFLP test?
And we will show them to the jury in a little bit, but would you please describe the frequency estimate for the match from among the RFLP probes that you tested 42A-3 against?
Yes. The actual loci that I tested, there were eight loci. Of these I used population data from six of the loci; specifically D1S7, D2S44, D4S139, D52110, D10S28 and D17579 and the results were as follows: The profile detected in this stain occurs in approximately one in 57 billion African Americans, one in 150 billion Caucasians and one in a hundred billion Hispanics, indicating that among randomly chosen individuals this RFLP profile is a rare event, and again, this is for unrelated individuals.
For the complete set of data that we routinely use in our laboratory, we don't have population data for the additional probes.
Your Honor, may the record reflect I'm going to put a patch in the RFLP column showing eight probes?
Now, Mr. Simpson, you have previously testified about a frequency estimate derived from the DQ-Alpha and D1S80 data. Is that still a valid estimate, at least as far as those tests are concerned?
What I would like to do is put a magnetic--blank magnetic cover over the PCR frequency data for 42A-3, and if you will, Mr. Sims, as you have in the other stains, would you please describe the more common to the least common and write them in on exhibit 262.
Okay. Let's move on to the Bundy rear gate stain. That was run on the same set of RFLP tests that 42A-3 was done; is that correct?
Did you obtain a similar number of RFLP matches as you did--with 117 as you did with 42A-3?
Your Honor, may the record reflect I have got an 8-probe patch that I'm going to place in the RFLP column for--on exhibit 259?
And I've got a similar magnetic blank patch that I'm going to place over the PCR data.
You previously provided DQ-Alpha and D1S80 frequency information for the PCR testing that you did on 117; is that correct?
And that data is still valid as it relates to the tests--the PCR tests that you performed; is that correct?
And what frequency estimate did you produce for the eight-probe match on 117 to Mr. Simpson's known reference sample?
These results are the same as what we obtained from the sock; namely, one in 57 billion African Americans, one in 150 billion Caucasians and one in a hundred billion Hispanics, again using the six loci there, could you write the frequency limit on that blank magnetic patch that is on exhibit 259.
KEY QUOTEOkay, Mr. Sims. Mr. Fairtlough, could I get the boards down and I would like to have marked as People's 400 for identification a set of eight autorads and I will describe--I've got them in a certain order here. They are labeled A-30. May that be 400-A; A-31, B; A-32, C; A-36, D; A-29, E; A-33, F; A-34, G; and A-35, H, your Honor.
At this point I would like to put on the elmo autorad A-30 which we have just had marked as 400-A for identification.
Mr. Sims, I'm going to ask you to come down here and use our point maker. Just generally speaking, though, before I ask you to show the jury how you have these things aligned, when did you actually begin the RFLP process on this set of stains that included sock A-3 and 117 and Mr. Simpson's reference sample?
And when did you--actually, have tests continued even beyond when you left Berkeley?
Yes. We--we at this point have the eight probes. We have one additional that has not been sized, but it came off just recently over the weekend and there is another one ongoing now.
Okay. About how much DNA did you use, for example, with sock stain A-3 or your 42A-3?
And how about with respect to 117, the rear gate stain? About how much DNA did you use?
Why don't we give you the point maker here. If you would, just help us get oriented on--from left to right, just so the jury can appreciate what they are looking at.
Okay. The--do I need to--I will go through this briefly because I remember we went through this last time. These are the ladders, what we call the ladders of the molecular size standard where you see several bands in particular lanes, so that would be here in lane 1, 4, 6, 8 and 10. Then as far as the sample lanes, we have in lane 2, this is that K562 sample. This is the national standard that is run as a control on everyone of our RFLP autorads. This third lane has our quality control sample. Again, this is the one that is unknown to me at the time of the analysis and I go ahead and do my sizing and submit the data to see whether or not I got the correct answer on that particular sample. Then skipping over here to lane 5, this is the lane in which we have the sock stain designated A-3, then another ladder, then we skip over here to Mr. Simpson, the Defendant's reference sample, his known blood sample in this lane, (Indicating). Another ladder and then in this next to the last lane we have the bloodstain no. 117 from the rear gate. And then we finally finish it off with another ladder sample.
Okay. Why don't we just mark the three samples, Mr. Simpson's sample, the sock sample and the rear gate sample, and this is the only one we will do it on, just so we can capture exactly what we have described. Could you use some color?
Yes, the sock is marked with pink. The Defendant's reference sample bands I will mark with the green.
Mr. Sims, I'm just going to ask you to point out where the bands--do you want me to hold that for you?
Just show the jury where the bands are that match among the three samples; Mr. Simpson's sample, the rear gate, 117 and sock A-3. I'm not going to ask you to mark them and capture them.
Okay. On the sock A-3 there is a band here, a band here, (Indicating). On the Defendant's reference sample again a band up here, (Indicating), a band here, (Indicating). And on no. 117, again high molecular weight band there and then the lower molecular weight band is down here, (Indicating).
Could you please point out where the bands that match among those three samples are.
Okay. For the sock A-3, the high molecular weight band is here, (Indicating), then a lower band is in this position. For the Defendant's reference sample, again a band up here, (Indicating), and then a lower band again in that position. And then for no. 117, the rear gate, the high molecular weight band is here, (Indicating), and the lower band is in that position again.
Your Honor, at this point I would like to bring in the light box and put all eight autorads up there, as well as 400-A, if that is okay.
Be careful. If you recollect, the box blew out our electricity the last time.
KEY QUOTEYour Honor, what I would like to do is put 400-A(1) up there along with the other autorads, the eight autorads.
Mr. Sims, in the order I've got these, would you place the eight autorads up there, as well as 400-A(1).
All right. Let the record reflect that all the jurors have had the opportunity and have taken the opportunity to review the eight autorads, People's 400.
Excuse me, Mr. Harmon. Do you want to step on the other side of the monster, please. And can we turn the lights off, please?
Mr. Sims, you actually had three stains from the rear gate that you did testing on; 115, 116 and 117; is that right?
From among those three stains the yields were lower on 115 and 116, such that it might be difficult to get any RFLP at all out of those two.
Okay. Just a couple other questions. On some of these--just to go back, because it has been a long time since the jury heard the description of the RFLP process, on some of these autorads there is something that looks like the San Andreas fault that goes across it?
There was a tear in part of that membrane on the side extending into the middle, around the middle of the actual membrane that occurred during the batch probing process.
It actually tore during the manipulation process, the handling process when it goes in and out of liquid or it is opened up, because it is contained in a plastic wrap.
Well, thanks to the skill of our batch probing team, they were able to put--it is not torn in half so they were able to bring the two edges right up against each other so that you were still able to go through the whole probing process. Theoretically you could cut this thing in pieces and still size it. It just makes life more difficult.
It was after the first probing, so it was after the D2 membrane was--autorad was obtained.
And that is the only one where you can't see this line that looks like an earthquake fault?
Actually I don't think you can see it in a lot of them. Some of them you can see it; most of them you can't, as I recall.
The profile detected in this stain occurs in approximately one in 57 billion African Americans, one in 150 billion Caucasians and one in a hundred billion Hispanics, indicating that among randomly chosen individuals this RFLP profile is a rare event.
These results are the same as what we obtained from the sock; namely, one in 57 billion African Americans, one in 150 billion Caucasians and one in a hundred billion Hispanics.
Because they had already been eliminated by the PCR results.
Be careful. If you recollect, the box blew out our electricity the last time.