Mr. Sims, you knew going into this process that the longest part of it would be the period of time that the probes were hybridizing?
You knew that that part of the process would take something on the order of fifteen days, sixteen days per probe, given the small amount of high-molecular weight human DNA you had?
Well, I think--I think it was in mid-May that I informed the court that I would need about two months.
All right. I'm giving you a very--I will make it easier for you. All right. By March 22nd you had done the quantitations on the combination and knew that you had 72 nanograms of high-molecular weight human DNA from 303, 304 and 305, right?
And then on April--by April 4th certainly you knew that that was 72 nanograms of high-molecular weight human DNA?
But it would be a fair statement that certainly by March 22nd you knew, if you only had 72 nanograms of at best human DNA, that the RFLP tests here would be longest in terms of elapsed time from the moment that that probe began to hybridize, right?
And if you wanted to get this done in a timely fashion before the end of the Prosecution's case, you had to get the probe started right away, correct?
Well, I don't think I had any idea of how long the Prosecution's case would be at that point in time.
Were you aware that when the court ordered that this combination could be--could take place, that it was the understanding stated in open court that this process of RFLP testing should be done immediately, if not sooner, quote-unquote?
Nobody communicated to you that this court said that it was its understanding that the RFLP process for the combined stains should begin immediately, if not sooner?
Well, I know that the--the Prosecution, which would be through Mr. Harmon, encouraged me to get this done as soon as I could, yes, but I don't recall the court ordering that--
In your discussions to get this done as soon as it could be, did you inform Mr. Harmon, as of June--March 22nd, when you knew you only had 72 nanograms of DNA, that the long part of this process would be the time that the probes began to hybridize?
I think he does, but I guess it is just for the purposes of the record. We all know he knows.
Now, in terms of this process--and I call your attention now to the questions that Mr. Harmon was asking you about the process that you prefer to do over three consecutive days involving the restriction, analytical gel and southern blot, now the restriction we all are talking about is putting that DNA into a test-tube with the restriction enzyme and cutting it up, right?
He was asking you questions about how you prefer to do it over three consecutive days, but you didn't even do this over three consecutive days?
I think that misstates my testimony. What I said is you can do the restriction separately and then set it aside. The three consecutive days are you do one day where you do the analytical gel overnight, the next day which is the electrophoresis, and then the next day is the southern blot overnight and then there is a third day where you have to dismantle the southern blot, so that is the three days that you need in a row that I spoke of.
All right. So in terms of the restriction, it is one hour's of hands-on work and then you leave it overnight?
Well, I would object to that characterization of one hour of hands-on work, because it is not just hands-on work; it is evaluating how much sample you have to put into this situation. In this particular case it is making sure we have enough sample. That sort of thing.
Wait a minute. I'm just--you just told us a second ago to do the restriction it took an hour of hands-on work. Just a second ago you said that--
Well, no. I agree with that being an hour of hands-on work, but I'm saying it is a lot more than hands-on work, that is my point.
That is my question, thank you. You let that sit over night and the analytical gel is two hours of hands-on work and then you let that go overnight?
All right. How many hours of hands-on work does it take you to put the DNA into the analytical gel, because that is what is at issue here?
Well, no. I don't mean to be argumentative, but I think you have to remember that after you've restricted the DNA, you have to do an evaluation, you run a digest gel, you have to then--
That is about a day's work, about a day's work after you do the restriction to get it now going overnight to do an analytical gel.
Mr. Sims, the southern blot, how long does it take you to prepare the southern blot? An hour and a half, two hours?
After the overnight electrophoresis, then it is about two hours of work to set up the southern blot.
All right. So you can do the restriction, the analytical gel and the southern blot, in what, three days?
We are now through that process, yes. There would be about three days, something like that.
All right. So it would take you--and part of that--it is not just three days of consecutive work. Part of that is you just have to let things go overnight?
All right. So before you get to the phase where there is this long elapsed time of letting the probe sit, you needed to do, essentially, work that would take three days to complete, right?
But in those three or four days, for example, if you had done the restriction, which you told us takes about a hour, and you let it sit overnight, you could have done the restriction in an hour and had a conversation with Mr. Harmon about discovery or preparation or other things, right?
Well, that is not really true, because a lot of these things are done at the end of the day. For example, the restriction is usually set up as the last thing that goes overnight, so it is not the sort of thing where I would do that the first thing in the morning and then later in the day talk to Mr. Harmon. That is not clear.
I have one last question, your Honor, that you might want to think about overnight, and this will be it.
From March 22nd when you knew that you had 72 nanograms of DNA and there would be a very extensive time period on the development of each probe, to the time that you went through what you described as the three or four-day process that would be necessary to get those probes in motion--right, that is the time period, okay?
So it is March 22nd and you began that process of getting those probes, that three or four-day preparation time to get the probes in motion, you began that on June 7th?
And it is your testimony that between March 22nd and June 7th you couldn't find three days to get that in motion so that the RFLP tests on the combined samples could be ready before the end of the Prosecution's case; is that right?
KEY QUOTEWell, it is the point that I did not find that. I came very close to doing this at the end of April, but I was not--I did not complete it at that time.
KEY QUOTEAnd you are not the only person that was doing--just so the Judge understands, in terms of these batch technicians, they are the ones that actually deal with the RFLP probes, technicians in your lab?
She didn't do any of these--didn't she do slot-blots and yield gels on some of these samples?
She did on some of the samples, but I don't believe she did any with regards to these particular samples, no.
And it is your testimony that between March 22nd and June 7th you couldn't find three days to get that in motion so that the RFLP tests on the combined samples could be ready before the end of the Prosecution's case; is that right?
Well, it is the point that I did not find that. I came very close to doing this at the end of April, but I was not--I did not complete it at that time.
You are saying it takes you a day to prepare the analytical gel. Come on?
The jury is out on that, too.