When we last left we were discussing areas, as you recall. I would like to first put up on the screen what has already been marked as Defendant's 1087, all right? Now, Mr. Sims, I direct your attention to the smear that--that you see where "30" is in this photograph?
Right. So starting to--as I'm looking at it and the jury is looking at it, all right, the upper left-hand--the--from the left going downward to the right, you see that streak where the arrow is pointing from there down to where the "30" is. All right?
Maybe we could have the arrow go there as well. And--and that area would be--starting at the top it is a little shadowed there, moving vertically down.
And let's assume that that 31 area is the same as 304. And now I would ask you to look at another photograph that we would ask be marked Defendant's next in order as 1167.
And I would like you to assume that last photograph I showed you, 1087, was a photograph taken on June 14th.
And I would like to--why don't you assume that 306 is just a--is a hair and is not a stain, okay?
And looking at the area again, 303, you see the same area that we pointed out on the June 14th photograph starting from the upper left-hand moving to the right, okay?
All right. I would like you to look at the area to the left of 303 and assume that is the same area as 30 on the June 14th photograph.
Objection, that is vague and it may be inconsistent with the testimony in this case as it is vague, your Honor.
All right. Now--you see an area below designated--the bottom of that designated "305"?
The stain collected as no. 30 which we have asked you to assume was done on June 14th, which I have already asked you to assume is the same area as the--let's just start this. 30 collected on June 14th, umm, you had a DQ-Alpha typing of 1.1, 1.2?
--you are talking over his answer again. The court reporters are almost in rebellion.
Then on June 14th, as far as 31 is concerned, which would be collected--31, you recall that stain?
All right. On June 14th you have a 1.1, 1.2 and what you are calling a weaker 1.3, 4, correct?
All right. But as far as on 304, which I'm asking you to assume was collected from the same area on September 1st--
And on the 304 stain collected on September 1st, now the list of people not excluded extends, in addition to Nicole Brown?
Although I would like to add, too, there was a little more definition I think in discriminating the mixture on the DQ-Alpha. You will notice it says weaker 1.3, 4, and on 305 as opposed to 304. The same alleles are present, but there is a little distinction there as far as the patterns.
In your examination of those photographs of the smears on--stains on June 14th and the photographs you saw of September 1st, could you tell whether there was more blood on the console on September 1st than there was on June 14th, just from looking at the photograph?
Yes, something like that. Again that may be after quantitation, but it is in that ballpark.
Now, in your opinion, as a forensic scientist, it is important to preserve the integrity of biological evidence whether it is found in an automobile or on a street?
And it is a good practice--withdrawn. Is it a good practice to permit individuals into an automobile for close to--for over two months before swatching the inside of an area for biological material?
Well, do you think that it would be a good practice, in your opinion of forensic scientists, to permit a car to be burglarized before you go in and swatch biological material from the area?
KEY QUOTEWell, do you agree that chain of custody principles apply to cars and their interiors, just like any other piece of evidence?
Now, looking just at the materials that I have asked you to assume were collected on June 14th, that would include 30 and 31 from the console, correct?
Now, with respect to 31, there was a problem, was there not, with the DQ-Alpha analysis of that stain?
I don't--I don't think there was a problem. I think that was what I would classify as a tough call. We had to take a hard look at it, but I don't think there was a problem with the analysis.
Before we examined what you have said was a tough call, would you not agree that with respect to the samples that were collected on June 14th, that there is no DQ-Alpha typing results of a 1.3 and a 4, putting aside 31, from any of the samples collected on June 14th, be it on the console or any other place in the Bronco?
There is no DQ-Alpha typing results of a 1.3 allele and a 4 allele from samples collected on June 14th, be it on the console or any other place in the Bronco, and I'm excluding here 31?
Do you recognize that as a photograph of DQ-Alpha strips for analyses you made of stains from the Bronco?
Do you see any differences between those photographs? Do you have a preference as to which one we use?
I--I would have to look at it for a minute. I recall Dr. Blake was present for this reading. I don't know if he took the photo at that exact time, but I think he did.
Would you, for purposes of analysis, and for being able to show this, do you have any problems, from a scientific point of view, using the photograph I have showed you, and please examine the two of them?
Now, maybe what we could do is you could have your photograph in front of us and we will put 1166 on the elmo. Okay. Now--
Now, let me call your attention to what is marked on the right-hand section of the strip, that is called dna-18.
All right. That is the strip where your--the one that up are characterizing as the tough call, correct?
And in terms of reading that strip, you see at the 1.3 dot a light dot--would that be--how would you describe that? Faint?
Well, I would describe it as a dot of about equal intensity to the c dot. When we talked about how important the c dot is and that intensity was scored on that day, that reading as c, and what we do when we do one of these strips is we score the dots in relationship to the c dot, so you first look at the intensity on the c dot and you call that C. It sounds very simple, but anyway, you call that c and then you Judge the relative intensities of the other dot, c plus or c minus, and we also have a category where we call them very faint or trace, so that was scored as a C.
What you are actually saying is that you feel confident that is right to the 1.3 in what is labeled dna-18, correct?
Yes. As far as our scoring was concerned, we considered that to be about the same intensity as the c dot.
Could a reasonable scientist look at that and say maybe it is a little less intense than the C dot?
I don't--I don't think it is really subjective in the sense that we are making an objective judgment about that intensity. We are also having a second person read it, so I don't--I don't think it is--it is subjective. I wouldn't use that definition.
Now, this would be one of those situations where the c dot could be lit up by the primary contributor?
And the c dot is not necessarily indicating anything about whether you are missing dots or alleles from other contributors?
Right. Now, I call your attention to--if we could move the--down a little bit--to the lane that is QC 816.
And I realize that it is hard to see on the elmo, but looking at the actual photograph of the dot-blot that you have--
--and looking at your own scoring of this, at the 1.3 dot you see a hint or a trace--
The--the--yes, I think on the--on that particular scoring, the first reader called it a hint. I called it an outline, which means it is even--in my mind even weaker than just a hint, but it is definitely a very, very, very faint reading.
It is hard to--your Honor, we are going to review this and then we are going to show the actual photograph to the jury. It can't be seen the other way.
Let me move back a second, and could you, Mr. Harris--actually, first moving back to--if you go up to dna-18--
Could you put an arrow by the 1.3 dot there. Up, further up. Right there, (Indicating), underneath that.
Okay. Now, let's move down--take the arrow down to the QC 816 and put it underneath the 1.3 dot. That is the 1.3 dot that you say--one reader scored as a hint and you scored as an outline on your quality assurance sample 816, correct?
All right. Now, the positive control is the sample that you run in every strip, correct, every run?
And the positive control in this case was scored as having a hint or a trace of the 1.3 dot?
Okay. Now, in your protocol you have a section that deals with what's known as the allelic control?
And the allelic control in this case would be what's known as the positive control on this run?
Well, in this--in this particular case. In other words, where we know the type, yes, that is the control for that.
Would you even say that the quality control sample, 816, would also be considered an allelic control for this run?
Well, no. I would consider that a blind because we don't know--the analyst does not know what the correct type of that sample is, so in other words, we have to make a determination and then we submit that for review to see whether or not we made the right call.
So--so in other words, in your protocol, when the term "Allelic control" is used, it is only referring to the positive control, not to QC 816?
I don't think we use the term "Allelic control" on the protocol. Can you show me specifically?
I sure do. That would make it faster. And this, Mr. Harmon, is at page 2119 of the materials. It is page 92 of your protocol and it is entitled "Controls for PCR analysis."
Okay. This is where we had a misunderstanding because this is the quality assurance manual. This is not the DQ-Alpha protocol.
I'm sorry, in your quality assurance manual you have a section that deals with allelic controls?
And the allelic controls would be referring to certainly the positive control here?
I--I wouldn't use that definition for it, but it is one of the controls we run. Again, it is blind, so you can't--the analyst doesn't know the correct type, so the analyst can't use that information to say, yes, this test is working properly and that is what a control is. That comes later with a review.
All right. So just dealing with the positive control then, in your protocol--you rely on this allelic control section of the protocol, do you not?
Okay. And in the allelic control section of your protocol does it not state: "This sample" and here we will be talking about the positive control that is depicted on the elmo--"Is a positive control that ensures that the amplification and typing process are working properly. To control for differential amplification the allelic control should include an allele that is sensitive to amplification conditions in this system. For DQ-Alpha a type 1.1, 4 control is to be amplified with each amplification run and typed with each set type," correct?
All that is saying is that that allelic control is what is known as the positive control here, correct?
And then at the end of this section of your protocol it goes on to state: "If the allelic control fails to give the correct result, the analysis must be repeated."
And in this instance the allelic control had showed evidence of a 1.3 dot, did it not?
No. I would say it showed that there was a cross-hybridization, very faint, at that particular location or at least a hint of it.
Well, again, I think when you say, "Lit up," in other words, do we see a hint of activity there? Yes, we do.
Okay. Would you not agree that with respect to the sample 31 or your dna-18, that the 1.3 dot in that sample is also certainly faint?
Your Honor, I move to strike this answer as not responsive. I asked him a simple question.
Now, as far as 1166 is concerned, could you please--your Honor, since these are faint, I would like the jury to see them. Could we mark on the lane that is dna-18 with an arrow, just point an arrow there and indicate--and after that indicate "1.3.".
And then on the sample 816, the quality control, could you put an arrow there and indicate--write down "1.3."
Overruled. It is merely to direct the viewer's attention to that location. Proceed.
Excuse me, counsel. Do you have my permission to ask another question before you hand--
Your Honor, I have been informed, for the record, I should say 1166-A is a printout.
Your Honor, while the jury is looking at it, maybe we can save some time by approaching on another exhibit that I have shown you.
Do you think that it would be a good practice, in your opinion of forensic scientists, to permit a car to be burglarized before you go in and swatch biological material from the area?
Yes, I have reviewed the notes and I did find more in 303 than 30.
If the allelic control fails to give the correct result, the analysis must be repeated.
I wouldn't score that as faint, no.
The court reporters are almost in rebellion.