Dr. Cotton, now, just again, because we had a little bit of a break, assuming again that the drops at Bundy were not deposited by Mr. Simpson, but were deposited by someone else, and assume that in the process of collection, processing and handling and packaging of those drops that the stains became so degraded as to lose their DNA content, to some extent, as it would look like here on item 56. I believe that also happened in your laboratory with respect to item 42, didn't it? That was the--from the pool of blood near Mrs. Nicole Simpson--Nicole Brown Simpson's body? Did you see that same phenomenon?
Well, put aside item 42 then, okay? I just would like you to make this assumption, that that degree of degradation occurred so that the items actually lost that DNA content.
I'm asking you to assume for the purpose of this hypothetical that the items, the swatches taken from the Bundy blood drops became so degraded as to lose all recognizable DNA. Okay?
Thank you. And I'm asking you to assume that at a later point in time, at the Los Angeles Police Department laboratory, they came into contact inadvertently with swatches from Rockingham that had Mr. Simpson's blood on it and that had more DNA in them. Okay?
I'm asking you to assume that they also came into contact with Mr. Simpson's reference sample, meaning the vial of fresh blood. All right? Just assume those three premises.
Sustained. As to that additional item, that's more than we discussed at sidebar. Sustained.
Okay. Fine. Limit the hypothetical then to the Bundy swatches coming into contact with the Rockingham swatches. All right?
Okay. Now, given those assumptions, is it possible scientifically that the--when they come into contact with Rockingham swatches, that they can be cross-contamination from Rockingham swatches to the Bundy swatches? Is that scientifically possible?
Are you asking me if it's scientifically possible or does the state of the DNA in this case support that?
Certainly I suppose it would be scientifically possible for a very small amount of material to be transferred from one swatch to another.
KEY QUOTEOkay. Now, Dr. Cotton, furthermore, we're talking about--oh, let me back up again. And if some of the material from the Rockingham swatches--
Excuse me. Mr. Neufeld, forgive me. Is--the autorads that are on top of the monitor here, are those evidence?
KEY QUOTEIf some of the blood from the Rockingham swatches that represent Mr. Simpson's blood came in contact with the swatches from Bundy which were degraded, so degraded as to not have any more DNA recognizable, given my hypothesis, my hypothetical--excuse me--under those facts and circumstances, Dr. Cotton, is it possible that the contaminated sample, contaminated by Mr. Simpson's blood is what you would see if you did a DNA test?
Okay. Well, in the first instance, let me ask you about item 52. Item 52 was a blood drop from Bundy; is that right?
And I think you said that based on your own experience in the laboratory, item 52 appears to have very little DNA in the lane; isn't that right?
And, in fact, you mentioned that there is a method that Cellmark has at its disposal to estimate the amount of human DNA in a sample; isn't that right?
It's referred to as a slot blot, which is just a way to use a probe that's human specific on a small part of the sample and see how much signal you get and compare that to a whole series of controls of known amounts, and you can make an estimation from that as about how much human DNA that you have. And it has some limitations, but it's very useful.
And did that slot blot produce a result giving you an estimate as to approximately how much DNA was present in the entire sample?
Okay. For this, I'm going to get out the original records since those will be better for me to be looking at.
While you're looking, Dr. Cotton, I'm just going to--with the Court's permission--replace the exhibit that's on the elmo with item 257-b, which reflects item 52.
Okay. Based on your review of the slot blot, is there an estimate as to how much DNA was present at least in the sample that you did the slot blot on?
From the slot blot, the amount of DNA appears to be about 25 nanograms altogether. However, the slot blot is a separate extraction from the DNA that was extracted that goes on the autorad. There is no slot blot for the DNA that was loaded on the agarose gel, which is what you're seeing on the autorad. The only estimation of that amount of DNA would come from the yield gel or looking at the film itself and making an estimation.
Well--okay. Let's just back up a second. The yield gel simply tells you how much DNA is present. It doesn't tell you whether or not it's bacterial DNA or human DNA; isn't that right?
And you're saying that this slot blot does not represent 10 percent of the entire sample you received minus that 10 percent cut that's saved?
All right. What percentage of the entire sample is represented in the slot blot approximately?
--and so that we won't get confused. 10 percent of the sample was appro--and this is an approximation because you're cutting from, you know, an irregularly shaped thing. 10 percent of the sample was used for the PCR test. The slot blot was done for that 10 per--a DNA extraction was done from that 10 percent. Of that DNA--for the PCR. And of that DNA extraction, 10 percent of it would have been loaded on the slot blot. So that all we can really say in response to your question is, of the DNA that was extracted for PCR, approximately 25 nanograms would have been available. That is maybe approximately 10 percent of the entire sample.
All right. Does--would you agree that the banding pattern--I'm sorry. Withdrawn. You said a moment ago that one way to estimate how much DNA is present in the sample is to look at the autorads themselves; is that correct?
And would you agree that there is significantly less DNA present in lane 52 than in any of the reference samples for instance?
We don't have a specific target, but generally we will not want to go below 25 nanograms.
Well, does your protocol say with respect to the question of how much DNA one can get a result from, say that 15 nanograms of DNA may give an RFLP banding pattern? Does it say that?
I don't have that huge protocol memorized. Uh, if that is a copy of it and you're reading from it, then I will agree with you. But--
KEY QUOTEBut what you're saying is that based on your experience, that sometimes you can even get an RFLP result from as little as 25 nanograms?
Yes. Actually you could probably even go slightly lower than that, but it depends on the quality of the DNA.
Okay. And would you agree that, as evidenced by the banding patterns here, you are close to that--that minimal amount that you're talking about?
Okay. And would you agree that oh, a microliter of blood has as little as 20 to 40 nanograms of DNA in it if it's in good shape?
To tell you the truth, I don't actually know what the correct figure for that would be.
Your Honor, I would do a whole series of questions if you'd like, and I'll do that now.
It's an organization of scientists who have done very important work in their respective fields and been elected to become members of the national academy of science.
Would it be fair to say that it basically includes the most distinguished members of the scientific community in this country?
Well, I don't know that it includes all of the most distinguished, but it includes a good many of them.
All right. And in 1990, did the national academy of science commission a study on the reliability of forensic DNA profile?
And did that study--to do that study, did the national academy of science appoint a committee to do the investigation?
A few more questions, counsel. I'll overrule it subject to later motion, but let's get to the chase here.
And would you agree that some of the more esteem members of the forensic science community and genetics community participated in this committee that eventually drafted a book?
I'll agree with you that some of the members were very knowledgeable, but you may have overstated their standing somewhat.
Okay. In fact, you personally testified before this committee, did you not, before this report was prepared?
Testimony wouldn't be my choice of words. I didn't take any kind of oath or anything. I just gave a short talk.
Okay. And to the best of your knowledge, this report was funded by in part the Federal Bureau of Investigation?
Uh, I believe that was where some of the funding came from and some came from other sources.
And eventually in April of 1992, the national academy of science press published their final report; isn't that right?
And no doubt, you have carefully--I think you even said on direct examination that you have read this report many times?
And whether or not you agree with all of its findings, Dr. Cotton, would it be fair to say that you have considered all that is written in this book before reaching your own opinions?
Have you considered the conclusions and observations in this book as part of the process of reaching your own opinions about the reliability of forensic DNA profiling?
Okay. And have you considered the chapter entitled "DNA typing technical considerations" as part of the process in reaching your own opinions and conclusions about forensic DNA profiling?
And now I'm going to ask you exactly the same question with respect to chapter 3, which is "DNA typing statistical basis for interpretation." have you thought about what they said as well?
And have you thought about what they said in chapter 4 entitled "ensuring high standards"?
Okay. Now, in that chapter 2, I would like you--what you said you did think about and consider in reaching your opinion and conclusions--
With respect to chapter 2 that you acknowledged the thinking about and considering, I would like to ask you about a particular table, table 1.1.
Dr. Cotton, and you said a moment ago that you thought about the contents of each of those specific chapters which I referred to. And did you consider the contents of those particular chapters which I just referred to in formulating or coming to your own conclusions and opinions whether or not you accept or reject the contents of those chapters?
Well, Dr. Cotton, did you consider the contents of chapter 2 in formulating your own opinions--
--in formulating your own opinions regarding the technical considerations for DNA typing?
I read that chapter. I thought about what it had to say. I don't agree with all the things that are in the chapter, but I certainly thought about them as I read them.
And when you thought about them as you read them, even if you didn't agree with--by the way, are there some things in the chapter that you do agree with?
In--that would be applied to the whole book. There are things that I agree with and there are things that I don't agree with.
But in helping you to reach your conclusions and your current opinions, did you at least consider the positions taken in those three chapters that we just described?
Counsel, I've indicated to you as clearly as I could, the issue is reliance in forming an opinion. The fact that you read something doesn't necessarily mean that you can be cross-examined on it. Has to be the basis of an opinion. Proceed.
KEY QUOTEIn forming your opinion that you've given to this jury in this case, did you consider the substance of what appears in chapter 2, "DNA typing technical considerations"?
If you're asking me, did I consider that chapter in forming my opinions that I've presented regarding this evidence, I have to say I did not.
KEY QUOTEWell, when I say that, what I--let me clarify that. Did you consider this chapter in formulating your opinions on the technical considerations of DNA typing in general, which of course is one of the things that you--well, let me back up. Are the technical considerations of DNA typing one factor that you consider when you assess evidence in a case?
And on a foundation or threshold level, Dr. Cotton, in coming to your own conclusions and understanding about the various technical considerations involved in forensic DNA profiling, did you consider what the national academy of science report said in this book, "DNA technology and forensic science"?
Certainly I suppose it would be scientifically possible for a very small amount of material to be transferred from one swatch to another.
If you're asking me, did I consider that chapter in forming my opinions that I've presented regarding this evidence, I have to say I did not.
Counsel, I've indicated to you as clearly as I could, the issue is reliance in forming an opinion. The fact that you read something doesn't necessarily mean that you can be cross-examined on it.
I don't have that huge protocol memorized.
Mr. Neufeld, forgive me. Is--the autorads that are on top of the monitor here, are those evidence? Okay. Just don't want you to melt them there. Proceed.