📄 Cross-examination of Dr. John Gerdes (part 1) — Monday, August 7, 1995
Address:
C:\DEPT103\CRIMINAL\1995\AUG\7\CROSS-EXAMINATION-OF-DR-JOHN-G.DOC
TRIAL
▲ Day 130 of 167

Cross-examination of Dr. John Gerdes (part 1)

Witness: Dr. John Gerdes
Examiner: George Clarke
Called by: Defense • Date: Monday, August 7, 1995 • Utterances: 797
Prosecutor George Clarke cross-examines defense DNA expert Dr. John Gerdes, methodically undermining his credibility using his own notes, grant proposal language, and prior testimony. Clarke then walks Gerdes through a comprehensive review of RFLP DNA results across the case, extracting concessions that multi-probe RFLP matches confirm the PCR results Gerdes had criticized — including an 8-probe match on the rear gate, a 14-probe match on the socks, and 12 total genetic markers matching Simpson on Bundy item 52.
1 (The following proceedings were held in open court, in the presence of the jury:)
2 THE COURT:

All right. Thank you, ladies and gentlemen. Please be seated. Let the record reflect that we have been rejoined by all the members of our jury panel. Good morning, ladies and gentlemen.

THE JURY: Good morning.

3 THE COURT:

Dr. Gerdes, would you resume the witness stand, please.

John Gerdes, the witness on the stand at the time of the evening adjournment, resumed the stand and testified further as follows:

4 THE COURT:

All right. The record should reflect that Dr. John Gerdes is on the witness stand undergoing cross-examination by Mr. Clarke. Good morning again, doctor.

5 DR. GERDES:

Good morning.

6 THE COURT:

Doctor, you are reminded, sir, you are still under oath. And Mr. Clarke, you may conclude your cross-examination.

7 MR. CLARKE:

Thank you, your Honor. Good morning, ladies and gentlemen.

THE JURY: Good morning.

CROSS-EXAMINATION (RESUMED) BY MR. CLARKE

8 MR. CLARKE:

Good morning, Dr. Gerdes.

9 DR. GERDES:

Good morning.

10 MR. CLARKE:

Dr. Gerdes, do you recall testifying last week that at the LAPD laboratory where DNA extraction is done that the laboratory does not use a laminar flow hood?

11 DR. GERDES:

Yes, that's correct.

12 MR. CLARKE:

Now, you visited the laboratory; is that correct, in December of `94?

13 DR. GERDES:

Yes.

14 MR. CLARKE:

And you took a tour of the lab, is that a fair description?

15 DR. GERDES:

Yes.

16 MR. CLARKE:

And that tour included the DNA laboratory, correct?

17 DR. GERDES:

Yes.

18 MR. CLARKE:

All right. Your Honor, with the Court's permission I would like to display to the witness exhibit 1301 which is a photograph of the laboratory.

19 (Brief pause.)
20 MR. CLARKE:

Dr. Gerdes, can you see that from that angle?

21 DR. GERDES:

Yes.

22 MR. CLARKE:

And in particular, with respect to this photograph, does this photo show the hood that you described during your testimony in the DNA lab at the LAPD?

23 DR. GERDES:

It does.

24 MR. CLARKE:

Now, as far as your tour of the laboratory back in December, did you make notes during that tour?

25 DR. GERDES:

Yes.

26 MR. CLARKE:

And in fact did you create rough, let's say, diagrams of various locations at the DNA laboratory at the Los Angeles Police Department?

27 DR. GERDES:

Yes.

28 MR. CLARKE:

Your diagrams included the area where DNA is extracted; is that right?

29 DR. GERDES:

Yes.

30 MR. CLARKE:

As well as where the hood is located?

31 DR. GERDES:

I believe so.

32 MR. CLARKE:

In terms of your keeping those notes, did you attempt to be as accurate as possible, setting aside exact scale?

33 DR. GERDES:

Yeah. There was no scale and it was just to give me a rough idea so that I could go back to those and recall what I had seen.

34 MR. CLARKE:

Now, did you have occasion to provide to the People last Friday notes that you took, including a diagram or diagrams of this particular tour?

35 DR. GERDES:

Yes MR. CLARKE: All right. Your Honor, I would ask to have marked as People's next in order, a single page, which I'm showing to counsel.

36 THE COURT:

569.

37 (Peo's 569 for id = 1-page document)
38 (Discussion held off the record between Defense counsel.)
39 MR. CLARKE:

May I approach the witness, your Honor?

40 THE COURT:

You may.

41 MR. CLARKE:

Dr. Gerdes, showing you People's exhibit 569, does that appear to be a Xerox copy of one of your notes?

42 DR. GERDES:

Yes.

43 MR. CLARKE:

And is that in fact the note that contains a rough sketch or rough diagram of the extraction area at the Los Angeles Police Department?

44 DR. GERDES:

Yes. These notes were taken on 12/19, the first visit.

45 MR. CLARKE:

December, 1994?

46 DR. GERDES:

Correct.

47 MR. CLARKE:

Your Honor, may I display a portion of this note also?

48 THE COURT:

You may.

49 (Brief pause.)
50 MR. CLARKE:

Now, I don't know, Dr. Gerdes, can you see the screen from there?

51 DR. GERDES:

Yes, I can.

52 MR. CLARKE:

Does this appear to be a--well, the lower portion of that single page of notes, People's exhibit 569?

53 DR. GERDES:

Yes.

54 MR. CLARKE:

And this is your rough sketch or rough diagram of the DNA extraction area at the LAPD?

55 DR. GERDES:

Yes.

56 MR. CLARKE:

Now, there appears to be a fairly long rectangle, comparatively speaking, in the middle of the diagram which is--I'm not sure I can really read it, but it looks like it has two words; is that right?

57 DR. GERDES:

Yes, it says "Sorting."

58 MR. CLARKE:

And?

59 DR. GERDES:

"Sorting bench" I believe is what it says.

60 MR. CLARKE:

Just to the left of where the arrow is located?

61 DR. GERDES:

Yes.

62 MR. CLARKE:

All right. Off to the left of that long rectangle towards the top there appears to be a square with the term "Hood" written inside?

63 DR. GERDES:

Yes.

64 MR. CLARKE:

Now, off to the left of that, what are the other to words that precede the word "Hood"?

65 DR. GERDES:

It says "Lam flow."

66 MR. CLARKE:

Meaning what?

67 DR. GERDES:

Well, at that time I hadn't really noticed that this wasn't a laminar flow hood. I put down "Laminar flow" and I didn't really realize until the second visit that this wasn't a laminar flow hood.

KEY QUOTE
68 MR. CLARKE:

Upon your initial visit to the Los Angeles Police Department you put down "Lam flow hood" to describe that particular hood?

69 DR. GERDES:

Yes. That is incorrect.

70 MR. CLARKE:

All right. Was that a mistake?

71 DR. GERDES:

It was--it was something that I checked later on and found to be a mistake, yes.

72 MR. CLARKE:

Now, Dr. Gerdes, you testified last week that your DQ-Alpha PCR typing is not ready for forensic testing, correct?

73 DR. GERDES:

In my opinion, yes.

74 MR. CLARKE:

And that continues to be your opinion today, correct?

75 DR. GERDES:

Until adequate controls are incorporated to control the contamination problem, that continues to be my opinion.

76 MR. CLARKE:

And it is your opinion that it should be used for either including potential donors of DNA or excluding?

77 MR. SCHECK:

Objection, asked and answered.

78 THE COURT:

Overruled.

79 DR. GERDES:

Yes.

80 MR. CLARKE:

Are you familiar with a method of typing what's called mitochondrial DNA?

81 DR. GERDES:

Yes.

82 MR. CLARKE:

Does that use PCR also?

83 DR. GERDES:

It does to produce a product that is unsequenced.

84 MR. CLARKE:

Okay. Without getting into great detail, does that method involved, after amplifying a sample, basically looking at the pieces of DNA information, these liters, A's, T's, G's and C's one by one?

85 DR. GERDES:

Yes.

86 MR. CLARKE:

Is that a method that is in place in forensic laboratories around the world?

87 DR. GERDES:

Not on a routine basis.

88 MR. CLARKE:

In your opinion is it ready for use in forensics?

89 DR. GERDES:

No.

90 MR. CLARKE:

You referred last week to a number of scientific publications discussing DQ-Alpha as, and I think you used the term "Demonstration papers"?

91 DR. GERDES:

In my opinion, that is what they are, yes.

92 MR. CLARKE:

Now, you have testified for approximately the last five years that PCR DQ-Alpha typing should not be used in forensics, correct?

93 DR. GERDES:

I've been very consistent in expressing my opinions about contamination and my concerns, yes.

94 MR. CLARKE:

And that has been for about five years?

95 DR. GERDES:

Yes.

96 MR. CLARKE:

You have actually come to courtrooms like this and testified the same way as far as that particular opinion?

97 DR. GERDES:

That is--

98 MR. CLARKE:

For the last five years?

99 DR. GERDES:

That's true.

100 MR. CLARKE:

As far as your own laboratory, is it correct that most of the methods that you use in your lab in Denver are methods that don't involve the use of kits?

101 DR. GERDES:

Most of the--no, that is not true.

102 MR. CLARKE:

Are most of the tests that you use tests that do involve the use of kits?

103 DR. GERDES:

Yes.

104 MR. CLARKE:

You mentioned chlymadia. That is one of the tests for a sexually transmitted disease that you use a kit that is actually manufactured by Roche, correct?

105 DR. GERDES:

That's correct.

106 MR. CLARKE:

What are the other tests that you use a kit in?

107 DR. GERDES:

Well, there is the vast majority of the testing our laboratory does; testing for antibodies for HIV, THLV, I can go on and on. It is probably a page-long list of things, most of which we do use FDA-approved kits.

108 MR. CLARKE:

Are any of these kits manufactured--well, let me rephrase. Were any of these kits developed in your own laboratory?

109 DR. GERDES:

No.

110 MR. CLARKE:

Is it then your testimony that most of your methods--well, let me strike that. Rephrase that if I may, your Honor. As far as a grant proposal that you described during your direct examination, do you recall that testimony?

111 DR. GERDES:

Yes.

112 MR. CLARKE:

This related to a grant that you have fairly recently received; is that right?

113 DR. GERDES:

Yes.

114 MR. CLARKE:

That related to a particular technique developed in your laboratory; is that right?

115 DR. GERDES:

It hasn't been developed yet. That is what the grant is for. We are proposing to develop that technology.

116 MR. CLARKE:

So the grant is basically your receiving, your laboratory, receiving money to develop a particular technique?

117 DR. GERDES:

Yes.

118 MR. CLARKE:

As far as that grant, does this process involve your laboratory submitting a proposal to a government agency?

119 DR. GERDES:

Yes.

120 MR. CLARKE:

And then the government--I'm sorry. And the government agency then uses that proposal to decide whether or not to grant your laboratory money to look at this proposed technique?

121 DR. GERDES:

That's correct.

122 MR. CLARKE:

As part of that proposal are you required to describe a number of different things in this application for money?

123 DR. GERDES:

Of course.

124 MR. CLARKE:

Including the technique that you wished to try to devise?

125 DR. GERDES:

That is true.

126 MR. CLARKE:

As well as information about the laboratory?

127 DR. GERDES:

Yes.

128 MR. CLARKE:

As well as information about the individuals who would be involved in developing this technique?

129 DR. GERDES:

That is true.

130 (Discussion held off the record between Deputy District Attorney and Defense counsel.)
131 MR. CLARKE:

Could I have a moment, your Honor?

132 (Discussion held off the record between Deputy District Attorney and Defense counsel.)
133 MR. CLARKE:

Dr. Gerdes, in this particular grant proposal--are you familiar with it?

134 DR. GERDES:

Of course.

135 MR. CLARKE:

And you have read the entire document?

136 DR. GERDES:

I wrote it.

137 MR. CLARKE:

You wrote the entire document? All right. Isn't it true that in that document you state, in reference to your laboratory: "Most of our testing could be described as home-brewed as opposed to commercial kits."

138 MR. SCHECK:

Your Honor, ask that the witness be shown the entire paragraph.

139 THE COURT:

Show him what page it is.

140 MR. CLARKE:

Dr. Gerdes, if I can refer you to what appears to be page 31 of the proposal.

141 DR. GERDES:

What part of this page are you at? Oh, here, I see it. Okay.

142 MR. CLARKE:

That statement that I just read, was that a statement that you wrote in that proposal?

143 DR. GERDES:

Yes. It refers to the fact that in our laboratory we do go through some research and development and then it is progressed onto testing that is routine, and it has to do with our research and development department. It has nothing to do with the testing menu that we offer at the present time, which is what you asked me initially.

144 MR. CLARKE:

Well, is it then correct that most of the testing methods in your laboratory are, quote, home-brewed?

145 DR. GERDES:

Most of the newer methods. This is talking about the next generation, if you will, from our laboratory. Most of what we do at the present time are kits that are FDA approved. What we hope to do is to move into the field of development of new diagnostic technologies, and we have been involved in that process over the--since I have been hired. That is one of my jobs. But we are not yet using those types of testing for routine testing.

146 MR. CLARKE:

What does "Home-brewed" mean?

147 DR. GERDES:

It simply means that you are developing the test yourself, as opposed to buying a kit.

148 MR. CLARKE:

You have described the chlymadia test kit as FDA approved, correct?

149 DR. GERDES:

Correct.

150 MR. CLARKE:

What are the other testing kits in case work that are FDA approved in your lab?

151 DR. GERDES:

For DNA?

152 MR. CLARKE:

Yes.

153 DR. GERDES:

That would be the only one for DNA. All of the kits that we use, other than that particular kit, involve serological methods.

154 MR. CLARKE:

Which don't involve testing DNA?

155 DR. GERDES:

Correct.

156 MR. CLARKE:

Could I have just a moment again, your Honor?

157 (Brief pause.)
158 (Discussion held off the record between the Deputy District Attorneys.)
159 MR. CLARKE:

Now, as far as degraded samples, Dr. Gerdes, and we spoke about that last week, your concern about the reliability of results obtained using PCR-based testing in forensics is based really on the nature of the specimen? In other words, the fact that it is a bloodstain that may have been on a sidewalk as opposed to blood removed from a patient in a hospital; is that correct?

160 DR. GERDES:

That is one of the aspects, yes.

161 MR. CLARKE:

Now, as far as this use of DQ-Alpha testing, are you familiar with its use on cases in which defendants have been, for instance, in prison for a number of years?

162 DR. GERDES:

Yes.

163 MR. CLARKE:

And in fact DQ-Alpha testing has been used to determine years later that a person may have been innocent of a crime; is that correct?

164 DR. GERDES:

It has been done for that, yes.

165 MR. CLARKE:

And in fact inmates have been released from prison as a result of the--I'm sorry--as a result of typing results obtained by using this PCR DQ-Alpha method?

166 DR. GERDES:

That's true.

167 MR. CLARKE:

Those cases may involve cases that occurred years earlier; is that correct?

168 DR. GERDES:

That's true.

169 MR. CLARKE:

Your concerns about degraded samples would apply equally to those cases as well; isn't that true, where the samples may be years old?

170 DR. GERDES:

Yes.

171 MR. CLARKE:

Is it your view that in those--under those circumstances that inmates should not be released from prison as a result of DQ-Alpha typing conducted what may be years later?

172 MR. SCHECK:

Objection, calls for a legal conclusion.

173 THE COURT:

Overruled.

174 DR. GERDES:

I think you have to take every case by case in terms of how much weight to put on that or whatever, but my position has always been consistent that until you have more adequate controls, and in specific in those kind of samples where they are old, degraded and in small quantities, this technology has a tremendous risk of error.

175 MR. CLARKE:

As far as the samples that were not amplified and typed at the Los Angeles department--and I asked you a number of questions of about that last week, correct?

176 DR. GERDES:

Excuse me. Go ahead.

177 MR. CLARKE:

Sure. As far as the samples that were not amplified and typed, in other words, they weren't subjected to this PCR process and typed at the LAPD, do you recall that?

178 DR. GERDES:

Yes.

179 MR. CLARKE:

Isn't it true that independent laboratories who reach the same results adds confidence to the results themselves? In other words, the layer or level of confidence that an analyst can have in the accuracy of those results?

180 DR. GERDES:

It will give you confidence from that step on, but what happens in the previous steps can't be cross-checked.

181 MR. CLARKE:

Isn't it correct, Dr. Gerdes, that if you work in two separate labs and try and come up independently with the same result, then that is always going to be a confirmation of the test and give you more reliability?

182 DR. GERDES:

Only if neither--both labs handled totally those specimens independently. That means they are collected and sent, as we have described earlier in the clinical situation, to both labs and are never exposed one lab to the other.

183 MR. CLARKE:

Haven't you previously testified to what I just stated, Dr. Gerdes?

184 DR. GERDES:

Of--I may have, but that was prior to more recent cases where it has become more and more obvious that there are problems early on.

185 MR. CLARKE:

Objection, move to strike. Nonresponsive, your Honor.

186 THE COURT:

Sustained. The last part of the answer is stricken. The jury is to disregard.

187 MR. CLARKE:

Dr. Gerdes, haven't you testified previously to what I just read to you?

188 DR. GERDES:

Yes.

189 MR. CLARKE:

Samples 48, 50 and 52, the bloodstains at Bundy, were swatches sent to different laboratories, correct?

190 DR. GERDES:

Correct.

191 MR. CLARKE:

As far as Cellmark is concerned--and I would like to shift your attention to known samples or if we use the term "Reference samples," can that indicate, as far as these questions, we are talking about known samples from the people who may be involved in a case, all right?

192 DR. GERDES:

Correct.

193 MR. CLARKE:

Referring in this case to Mr. Simpson?

194 DR. GERDES:

Yes.

195 MR. CLARKE:

As well as Nicole Brown and Ronald Goldman?

196 DR. GERDES:

Yes.

197 MR. CLARKE:

Isn't it correct that when Cellmark conducted its testing on the known samples they were done blindly?

198 DR. GERDES:

They were coded, yes.

199 MR. CLARKE:

And by "Coded," what do you mean?

200 DR. GERDES:

They were given a code number when they arrived at the laboratory and then the technician would type it from there.

201 MR. CLARKE:

Well, in addition--

202 DR. GERDES:

So blinded if you want to call it that, yes.

203 MR. CLARKE:

In addition to having a code number, isn't it correct that the actual known reference samples weren't labeled for Cellmark's purposes as to who of the three people they came from?

204 DR. GERDES:

As they received them?

205 MR. CLARKE:

Yes.

206 DR. GERDES:

I don't recall if that is the case. I believe--I know they were labeled with a code name, but I'm not sure if they will have the information as to who they he were from. I would have to double-check.

207 MR. CLARKE:

All right. Do you have information at your hand would be able--I'm sorry--help you answer that question?

208 DR. GERDES:

Perhaps--perhaps I do.

209 (Discussion held off the record between the Deputy District Attorneys.)
210 DR. GERDES:

That appears to be correct. They were labeled with a code name and it doesn't have who it is next to it.

211 MR. CLARKE:

So Cellmark was in fact given these known samples and they were coded C-1, C-2 and C-3, weren't they?

212 DR. GERDES:

That's correct.

213 MR. CLARKE:

And they were not given information about which sample was Mr. Simpson's?

214 DR. GERDES:

That's correct.

215 MR. CLARKE:

Which sample was Mr. Goldman's?

216 DR. GERDES:

That's correct.

217 MR. CLARKE:

And what sample was Nicole Brown's?

218 DR. GERDES:

Correct.

219 MR. CLARKE:

In your opinion that is an important step?

220 DR. GERDES:

I think that is the way to do it, yes.

221 MR. CLARKE:

Isn't it also correct that as far as the DQ-Alpha types that the Department of Justice typed that those reference samples were done after most of the evidence was typed?

222 DR. GERDES:

I believe that is true.

223 MR. CLARKE:

And in fact isn't it true that the known samples were not typed until after there were PCR results on several of the Bundy blood drops at the Department of Justice?

224 DR. GERDES:

I don't recall if that is specifically the case, but that is possible.

225 MR. CLARKE:

After several of the stains from the Bronco were tested by the Department of Justice?

226 DR. GERDES:

That is possible.

227 MR. CLARKE:

Well, isn't it true?

228 DR. GERDES:

I don't recall. I can look it up if you would like and try and look that up.

229 MR. CLARKE:

Is that something you think you can find in your notes?

230 DR. GERDES:

I think I can.

231 MR. CLARKE:

All right.

232 (Brief pause.)
233 DR. GERDES:

That appears to be the case, yes.

234 MR. CLARKE:

Those reference samples were typed, after, for instance, one of the blood trail at Rockingham was typed, one of those drops also?

235 DR. GERDES:

That's correct. That's correct.

236 MR. CLARKE:

As well as most of the samples on the glove?

237 DR. GERDES:

Yes.

238 MR. CLARKE:

As well as most of the samples on the socks as well?

239 DR. GERDES:

At DOJ, yes.

240 MR. CLARKE:

In your view is that also an important step to take, to type those reference samples after evidence is typed?

241 DR. GERDES:

I think that was appropriate.

242 MR. CLARKE:

Now, I would like to return, if I could, Dr. Gerdes, to the fact that in your laboratory you develop methods for use in DNA typing in your lab, correct?

243 DR. GERDES:

Correct.

244 MR. CLARKE:

Part of your function at the laboratory--and I believe you said you are director of the research and development arm of the lab?

245 DR. GERDES:

That is one of the titles I have, yes.

246 MR. CLARKE:

And in that role, as far as the research and development role of the laboratory, you try to develop methods that other laboratories can use, correct?

247 DR. GERDES:

Yes.

248 MR. CLARKE:

And in fact your proposal for a grant is one such proposal, to develop a specific technique to type DNA that other laboratories can use?

249 DR. GERDES:

Hopefully, yes.

250 MR. CLARKE:

You want to make sure that your techniques operate properly before they are used, for instance, in clinical case work?

251 DR. GERDES:

Of course.

252 MR. CLARKE:

That is absolutely essential, isn't it?

253 DR. GERDES:

Of course.

254 MR. CLARKE:

You also want to be able to market or sell these methods as quickly as possible; isn't that correct?

255 DR. GERDES:

Yes.

256 MR. CLARKE:

Is your laboratory committed to rapidly implementing DNA technology to diagnostic testing?

257 DR. GERDES:

We--yes.

258 MR. CLARKE:

And in fact you want to get these new techniques out there and marketed as soon as possible, correct?

259 DR. GERDES:

We have a projection as to how long this will take. The technology we are describing is so new it is going to take three to five years before we even confirm the technology itself, but we are really not talking about marketing for that many years.

260 MR. CLARKE:

Well, Dr. Gerdes, isn't it true that your laboratory is committed to getting these newly developed techniques into the clinical laboratory as quickly as possible?

261 DR. GERDES:

We would--we would like that, yes.

262 MR. CLARKE:

And in fact that is what you told the United States government as far as your grant proposal, didn't you?

263 DR. GERDES:

Yes.

264 MR. CLARKE:

As far as--we've talked about PCR as one technique. There is also the technique of RFLP that you have discussed in your direct examination, correct?

265 DR. GERDES:

Yes.

266 MR. CLARKE:

Can we call those different methods or methodologies? What word would you feel comfortable with to describe that there are two different techniques?

267 DR. GERDES:

Different methods is fine.

268 MR. CLARKE:

Isn't it correct that a consistency in result, that is a result is giving the same answer from both PCR testing as well as RFLP testing on the same sample, is one measure of the accuracy of the PCR technique?

269 DR. GERDES:

It does tell you some information. The RFLP is a more discriminating test, so you would expect that there would be a number of occasions where the PCR would include someone and RFLP would exclude them.

270 MR. CLARKE:

Okay. And that is certainly one characteristic of RFLP typing, that it is more powerful in telling people apart, correct?

271 DR. GERDES:

Correct.

272 MR. CLARKE:

If you saw PCR results that, for instance, exclude someone and from the same sample an RFLP included the person, that would give you great pause for concern about PCR typing, wouldn't it?

273 DR. GERDES:

Not necessarily. It depends on how many probes and in any genetic analysis you look at multiple markers, and once you find an exclusion, if it is confirmed, especially, then you have to believe that.

274 MR. CLARKE:

Well, let's make it more specific. Let's say that on a particular sample you saw a five-probe RFLP match. That would be powerful evidence of who that sample came from, wouldn't it?

275 DR. GERDES:

It would.

276 MR. SCHECK:

Objection, vague.

277 THE COURT:

Overruled.

278 MR. CLARKE:

If you saw a PCR result on the same sample that revealed results that excluded that person, wouldn't that give you concern about the PCR result?

279 DR. GERDES:

In a forensic setting, yes.

280 MR. CLARKE:

So therefore if there is consistency between the RFLP results and PCR results, doesn't that give you greater confidence in the PCR results?

281 DR. GERDES:

It can.

282 MR. CLARKE:

Well, haven't you testified previously, Dr. Gerdes, that you have no problem with the RFLP technology?

283 DR. GERDES:

I have.

284 MR. CLARKE:

And you have testified in this case that you have no problem with several of the RFLP matches in this case; isn't that true?

285 DR. GERDES:

That's right.

286 MR. CLARKE:

Isn't it in fact the case that if there is an RFLP match on a sample, in your opinion that validates the PCR result on that sample?

287 DR. GERDES:

It can.

288 MR. CLARKE:

Haven't you previously testified that it does?

289 DR. GERDES:

I probably have in the past, yes.

290 MR. CLARKE:

Isn't it true that if there is an RFLP match on the same sample that a PCR result is obtained, that that RFLP match confirms the PCR result on that sample?

291 DR. GERDES:

Of course you have to look at the sample. Even in RFLP there are going to be circumstances where those kind of results need to be looked at with suspicion in terms of whether it is a mixed sample and the same sorts of issues that we discussed earlier in general in terms of DNA technology and forensics, so you can't make a broad blanket general statement like that. You need to look at the circumstances for each item even in an RFLP test.

292 MR. CLARKE:

Dr. Gerdes, haven't you previously testified that if you get a PCR result on a sample and you already have an RFLP result on that sample, that that RFLP result confirms the PCR result?

293 DR. GERDES:

Assuming there are no difficulties or suspicions about the nature of the sample or mixtures or other complications like that.

294 MR. CLARKE:

Isn't it true, doctor, that if you cross-check the PCR results and RFLP results on enough samples in a given case that that is a significant method of validation?

295 DR. GERDES:

I wouldn't call it validation.

296 MR. CLARKE:

Haven't you used those exact words, Dr. Gerdes?

297 DR. GERDES:

I believe maybe three years ago I might have.

298 MR. CLARKE:

And in fact those words that you used were just as I stated to you, were they not, that if you cross-check the PCR results and RFLP results in a given case on enough samples that that is in fact a significant method of validating the accuracy of the PCR results?

299 DR. GERDES:

I probably said that, but you have to look at the context around that. I'm sure I also said that you have to look at the sample and the things that I've talked about here.

KEY QUOTE
300 MR. CLARKE:

When you said it, did you mean it?

301 DR. GERDES:

Yes.

302 MR. CLARKE:

May I have a moment, your Honor?

303 (Brief pause.)
304 (Discussion held off the record between the Deputy District Attorneys.)
305 MR. CLARKE:

Dr. Gerdes, didn't you in fact testify to what I just read to you less than one year ago?

306 DR. GERDES:

I don't remember how long ago it was.

307 MR. SCHECK:

Your Honor, I think that we have--should have an opportunity to see what he is referring to. We thought those were the rules.

308 THE COURT:

Overruled at this point, but if you are going to confront him with some specifics, we need to see it.

309 MR. CLARKE:

I understand.

310 MR. CLARKE:

Do you recall testifying in a case involving a Defendant named McIntosh in the state of California?

311 DR. GERDES:

Yes, I do.

312 MR. CLARKE:

Isn't it correct that in that case you testified to the fact that if there is this cross-check on enough samples that that is a significant method of validation?

313 MR. SCHECK:

Objection, needs to be confronted with the specifics.

314 THE COURT:

Objection. The form of the question.

315 MR. CLARKE:

Dr. Gerdes, with regard to this McIntosh case, didn't you testify in that case under a year ago?

316 DR. GERDES:

Yes.

317 MR. CLARKE:

Isn't it in that case that you made the specific comments that I just read to you?

318 DR. GERDES:

I may have.

319 MR. SCHECK:

Your Honor, I have--

320 THE COURT:

Counsel, do you have volume, line, page?

321 (Discussion held off the record between the Deputy District Attorneys.)
322 MR. CLARKE:

Can we approach the bench?

323 THE COURT:

You need to have that.

324 (Nods head up and down.)
325 (Discussion held off the record between the Deputy District Attorneys.)
326 MR. CLARKE:

May I proceed and return to that, your Honor?

327 MR. SCHECK:

Your Honor, I'm going to move to strike unless he can show the specific reference.

328 THE COURT:

Overruled. Proceed.

329 MR. CLARKE:

Thank you.

330 THE COURT:

It will be subject to a motion to strike, though.

331 (Discussion held off the record between the Deputy District Attorneys.)
332 MR. CLARKE:

In your view, Dr. Gerdes, is a 5-probe RFLP match a significant match?

333 DR. GERDES:

Yes.

334 MR. CLARKE:

What about a 6-probe match? Is that significant?

335 DR. GERDES:

Yes.

336 MR. CLARKE:

An 8-probe match?

337 DR. GERDES:

Yes.

338 MR. CLARKE:

Is a 14-probe match on a sample significant as far as RFLP typing?

339 DR. GERDES:

Yes.

340 MR. CLARKE:

Is a 14-probe match significant whether it is PCR or RFLP on a sample?

341 DR. GERDES:

It depends on the samples. We need to--again, you know, all of these are significant in terms of the number of matches, but you have to, of course, take into consideration the nature of the sample, whether there is a mixture or not. There are other things that complicate this issue. I can't just give you a blanket statement that that many probes is significant unless I know the nature of the samples and whether they are mixtures and those sorts of issues.

342 MR. CLARKE:

If you see a sample that is a 6-probe match that is showing either one or two bands that clearly match the one or two bands that an individual has, that is a significant match, isn't it?

343 MR. SCHECK:

Objection, "Validated."

344 THE COURT:

Overruled.

345 DR. GERDES:

Yes.

346 MR. CLARKE:

Whether it is eight or fourteen, if six is significant, anything more is significant; is that right?

347 DR. GERDES:

That's true.

348 MR. CLARKE:

As far as this case did you examine the RFLP results obtained by the laboratories in this case?

349 DR. GERDES:

I did.

350 MR. CLARKE:

And you are familiar with them?

351 DR. GERDES:

Yes.

352 MR. CLARKE:

Isn't it correct, Dr. Gerdes, that as far as the RFLP and PCR results in this case that the RFLP results confirm the PCR results in this case?

353 DR. GERDES:

In certain items, yes.

354 MR. CLARKE:

It is true on a number of items, isn't it?

355 DR. GERDES:

Yes.

356 MR. CLARKE:

And in fact this case involves many RFLP matches, doesn't it?

357 DR. GERDES:

Yes.

358 MR. CLARKE:

Have you seen a case with more RFLP matches in your history of consulting with criminal defendants?

359 MR. SCHECK:

Objection.

360 THE COURT:

Sustained.

361 MR. CLARKE:

Your Honor, at this time I have a board that I would like to be marked as People's next in order.

362 THE COURT:

570--570.

363 (Peo's 570 for id = posterboard)
364 MR. CLARKE:

570?

365 THE COURT:

570.

366 (Brief pause.)
367 MR. CLARKE:

Actually would it--I think in the normal place if that is acceptable.

368 DR. GERDES:

May I step down?

369 MR. CLARKE:

Yes, please, doctor. Thank you.

370 (Witness complies.)
371 MR. CLARKE:

Now, Dr. Gerdes, I would like to show you what has been marked People's exhibit 570, I believe.

372 THE COURT:

570.

373 MR. CLARKE:

This board is entitled at the top "Results of RFLP DNA analysis"; is that right?

374 DR. GERDES:

Yes.

375 MR. CLARKE:

And then there appear to be a number of different samples listed on the far left; is that right?

376 DR. GERDES:

Yes.

377 MR. CLARKE:

And then across on the right are columns entitled basically which laboratory produced the result?

378 DR. GERDES:

Yes.

379 MR. CLARKE:

Followed by what the RFLP results were in terms of the number of probes?

380 DR. GERDES:

Yes.

381 MR. CLARKE:

And then lastly, individuals who are not excluded from those particular samples as having been the donors of the DNA; is that right?

382 DR. GERDES:

That's correct.

383 MR. CLARKE:

Now, turning your attention first, there appear to be four samples from LAPD item no. 9, the glove; is that correct?

384 DR. GERDES:

Yes.

385 MR. CLARKE:

And on those--and let's start with the first sample and can we use G1? Is that a way of telling the four samples apart?

386 DR. GERDES:

Yes.

387 MR. CLARKE:

That sample revealed results from the Department of Justice after testing at five RFLP probes, correct?

388 DR. GERDES:

Correct.

389 MR. CLARKE:

And those results excluded, that is, indicated a mixture of Nicole Brown and Ronald Goldman, correct?

390 DR. GERDES:

Correct.

391 MR. CLARKE:

In your testimony you have not criticized the accuracy of that result, correct?

392 DR. GERDES:

That's correct.

393 MR. CLARKE:

And in fact in your testimony you have conceded that that result is accurate?

394 DR. GERDES:

Yes.

395 MR. CLARKE:

Incidentally, when you use the term, what does a significant match mean? I'm not sure that question was clear. Let me rephrase it. As far as a 5-probe match or a 6-probe match and so on being a significant match, what does the term "Significant" mean in that context to you?

396 DR. GERDES:

Umm, as you know, there is a controversy as to how--you know, what level of significance there is, but in my opinion five probes would be certainly very significant in terms of putting some weight as to that match.

397 MR. CLARKE:

And in fact in your opinion that would be strong evidence that in fact a sample that matches at five probes came from a particular individual who matches that sample?

398 DR. GERDES:

Again, it is controversial and I am not a population biologist, I am not involved in that controversy, but that is--it would be, in my opinion, significant.

399 MR. CLARKE:

When you perform a paternity analysis how many probes do you routinely use in determining whether or not a father is the father of a child?

400 DR. GERDES:

Paternity you are comparing apples and oranges. It is different statistics, it is done in an entirely different way, so it is really not a good comparison, but we do use five probes.

401 MR. CLARKE:

Five probes?

402 DR. GERDES:

Yeah.

403 MR. CLARKE:

Would that be the same number as were used on at least item G1?

404 DR. GERDES:

Yes.

405 MR. CLARKE:

All right. Turning to G2, those results reflected a 5-probe RFLP match to both Nicole Brown and Ronald Goldman, correct?

406 DR. GERDES:

Correct.

407 MR. CLARKE:

And you have no scientific criticism of that result?

408 DR. GERDES:

No.

409 MR. CLARKE:

Turning to G3, that result revealed an 8-probe match to Ronald Goldman, correct?

410 DR. GERDES:

Yes.

411 MR. CLARKE:

And in your view that result was appropriate, correct?

412 DR. GERDES:

Yes.

413 MR. CLARKE:

And then lastly on the gloved area, G4, the results showed a 5-probe RFLP match to both Ronald Goldman and Nicole Brown, correct?

414 DR. GERDES:

Correct.

415 MR. CLARKE:

No criticism of that result?

416 DR. GERDES:

That's correct.

417 MR. CLARKE:

Turning to the Rockingham foyer, item no. 12, Cellmark produced a 5-probe match to Mr. Simpson using RFLP, correct?

418 DR. GERDES:

Correct.

419 MR. CLARKE:

You don't have any criticism of the accuracy of the results obtained by Cellmark, correct?

420 DR. GERDES:

That's correct.

421 MR. CLARKE:

Turning to the sock, in fact there were a total of, and let's start with the ankle area, both the Department of Justice and Cellmark tested the ankle area of the sock using RFLP, correct?

422 DR. GERDES:

Correct.

423 MR. CLARKE:

That testing revealed at the Department of Justice an 11-probe match to Nicole Brown, correct?

424 DR. GERDES:

Correct.

425 MR. CLARKE:

Cellmark also probed staining from that same sample at five probes; is that right?

426 DR. GERDES:

Correct.

427 MR. CLARKE:

How many probes does that total?

428 DR. GERDES:

Sixteen.

429 MR. CLARKE:

Is that then a 16-probe with Nicole Brown?

430 DR. GERDES:

It is.

431 MR. CLARKE:

If two of the probes used by Cellmark and DOJ were the same, would it then be a 14-probe match in reality?

432 DR. GERDES:

Yes.

433 MR. CLARKE:

And that is in fact the case, isn't it, Dr. Gerdes?

434 DR. GERDES:

Yes, yes.

435 MR. CLARKE:

That two of the probes used by those laboratories are the same?

436 DR. GERDES:

Yes.

437 MR. CLARKE:

You have no quarrel with the accuracy of the results on the sock; is that right?

438 DR. GERDES:

That's correct.

439 MR. CLARKE:

Turning to the Bundy walkway, item no. 52, Cellmark produced a 5-probe match with Mr. Simpson, correct?

440 DR. GERDES:

Correct.

441 MR. CLARKE:

And in fact that data correctly reveals a 5-probe match with Mr. Simpson, correct?

442 DR. GERDES:

In this particular item there is very little DNA and in this particular item I disagree with that.

443 MR. CLARKE:

Is it your opinion that Dr. Cotton was incorrect when she concluded that that match existed with regard to Mr. Simpson?

444 DR. GERDES:

She is not incorrect in concluding the match, but the source of the DNA here is what the issue is. That is where the question is. There is very little DNA. It is down at the level of DNA where cross-contamination could be the explanation and the circumstances under which that particular item was handled makes that a distinction possibility.

445 MR. CLARKE:

Dr. Gerdes, is it your testimony that the RFLP results on item no. 52 came from contamination?

446 DR. GERDES:

Yes.

447 MR. CLARKE:

And in fact is it your opinion to a reasonable scientific certainty that item 52 revealed DNA that was not from the donor of the bloodstain but in fact came from another source?

448 DR. GERDES:

I think it is consistent with that.

449 MR. CLARKE:

You are not offering an opinion that it came, but you are raising the possibility; is that correct?

450 DR. GERDES:

That's correct.

451 MR. CLARKE:

Turning to item no. 78, the drop that revealed a 5-probe match to Nicole Brown and Ronald Goldman, correct?

452 DR. GERDES:

Correct.

453 MR. CLARKE:

And then lastly on this board, referring to item no. 117, the Bundy rear gate, first of all, how many probes--there doesn't appear to be anything written in under "RFLP results," correct?

454 DR. GERDES:

Correct.

455 MR. CLARKE:

Can you tell us how many probes that sample matched Mr. Simpson at?

456 DR. GERDES:

I don't recall offhand. At least five, but I'm not sure how many they--

457 MR. CLARKE:

All right. Is that something that you can determine from your notes?

458 DR. GERDES:

I did not bring the RFLP notes with me.

459 MR. CLARKE:

Are they available in the courtroom?

460 (No audible response.)
461 MR. CLARKE:

As far as your notes?

462 DR. GERDES:

My notes, no.

463 MR. CLARKE:

Yes. All right.

464 MR. SCHECK:

I could suggest--

465 MR. CLARKE:

I'm sorry.

466 MR. SCHECK:

--the results board has them.

467 THE COURT:

Proceed.

468 MR. CLARKE:

Object to counsel's comments.

469 THE COURT:

Proceed.

470 MR. CLARKE:

Dr. Gerdes, are you guessing it was at five probes?

471 DR. GERDES:

Until I look that up I don't know exactly how many probes there were on that particular item.

472 MR. CLARKE:

Your testimony has revealed you have no criticism of that match to Mr. Simpson; is that correct?

473 DR. GERDES:

That's correct.

474 MR. CLARKE:

All right. Thank you, your Honor.

475 (Brief pause.)
476 MR. CLARKE:

Incidentally, Dr. Gerdes, you are not familiar with the fact that there were serology tests conducted on many of these evidence items, correct?

477 MR. SCHECK:

Asked and answered.

478 THE COURT:

We have gone over this.

479 MR. CLARKE:

Merely foundational to the next question.

480 THE COURT:

All right. Proceed.

481 DR. GERDES:

I'm not familiar with the--I have not seen the reports. I am familiar that they were done, but I'm not familiar with the specifics.

482 MR. CLARKE:

Isn't it true that if the serology results confirmed--well, let me rephrase. Isn't it true that if the serology results were consistent with the PCR results that serology would be another methodology that the accuracy of the PCR results can be cross-checked against?

483 MR. SCHECK:

Objection, asked and answered, beyond the scope.

484 THE COURT:

Sustained. You have already asked that question.

485 MR. CLARKE:

Do you utilize serology to confirm the accuracy of any of the results in your own laboratory?

486 DR. GERDES:

It is the other way around. We have serological tests where we may do PCR to confirm that.

487 MR. CLARKE:

So you use PCR to confirm the accuracy of your serology results?

488 DR. GERDES:

In certain circumstances.

489 MR. CLARKE:

Incidentally, serologic techniques aren't nearly as sensitive as PCR, correct?

490 DR. GERDES:

That's correct.

491 MR. CLARKE:

It would be much harder to cross-contaminate a sample and obtain serological results than it would a PCR result, correct?

492 DR. GERDES:

In general that is true, yes.

493 MR. CLARKE:

In other words, cross-contamination isn't the same concern with serologic techniques because they are not as sensitive as PCR is to detect small amounts?

494 DR. GERDES:

It depends--you have to look at the specific test and so forth, but in general that is true.

495 MR. CLARKE:

As far as cross-checking by these different techniques, PCR and RFLP, isn't it true that additional markers beyond DQ-Alpha, such as polymarker, the five polymarkers and D1S80, give the analyst an opportunity to cross-check the accuracy of results even more?

496 DR. GERDES:

Again, you have to talk about the specifics and in the polymarker system there is a 2-probe--in most cases there are two allele systems. You know, two of them are three-allele and the rest are two-allele systems, and if you are dealing with mixtures, such as a contaminant would introduce, it is frequently not informative because you have only two, you have an a and a B, and if you have both, then that includes everybody, so it is not quite as informative as DQ-Alpha being able to look at the possibility of additional human DNA being there.

497 MR. CLARKE:

In other words, as to the polymarkers, they don't have a lot of different variations, correct?

498 DR. GERDES:

Right.

499 MR. CLARKE:

Individually?

500 DR. GERDES:

That's correct.

501 MR. CLARKE:

But they can present very informative results when you look at many of them, such as the five?

502 DR. GERDES:

It is possible, yes.

503 MR. CLARKE:

D1S80 is another marker, correct?

504 DR. GERDES:

Yes.

505 MR. CLARKE:

And in fact it has more variations or different possible types than the five polymarkers?

506 DR. GERDES:

Yes.

507 (Discussion held off the record between the Deputy District Attorneys.)
508 MR. CLARKE:

Your Honor, I would like, with the Court's permission, to use People's exhibit 259, the Bundy results board.

509 (Brief pause.)
510 MR. CLARKE:

Now, Dr. Gerdes, have you had an opportunity to see either this board or a board containing similar information?

511 DR. GERDES:

Yes.

512 MR. CLARKE:

All right. And I would like to start, if I could, with what is labeled at the top "Item no. 47, first drop by victims."

513 DR. GERDES:

Yes.

514 MR. CLARKE:

That particular sample was typed using PCR, correct?

515 DR. GERDES:

It was.

516 MR. CLARKE:

And how many different genetic markers produced a result consistent with Mr. Simpson?

517 DR. GERDES:

Well, there were the--the DQ-Alpha, D1S80 and the polymarkers.

518 MR. CLARKE:

Which would be how many total genetic markers?

519 DR. GERDES:

Seven.

520 MR. CLARKE:

Turning your attention to item 48, how many genetic markers produced a result on that particular item from the Bundy walkway that were consistent with Mr. Simpson?

521 DR. GERDES:

Seven.

522 MR. CLARKE:

Same seven genetic markers?

523 DR. GERDES:

Yes.

524 MR. CLARKE:

Turning now to item 49, how many genetic markers produced that result using PCR consistent with Mr. Simpson?

525 DR. GERDES:

They didn't get a D1S80, so that would be six.

526 MR. CLARKE:

Turning to item no. 50, how many PCR-based genetic markers produced that sample result consistent with Mr. Simpson?

527 DR. GERDES:

Seven.

528 MR. CLARKE:

Turning to item no. 52, there were five RFLP probes consistent with Mr. Simpson?

529 DR. GERDES:

Yes.

530 MR. CLARKE:

Were there also PCR markers that produced results consistent with Mr. Simpson?

531 DR. GERDES:

Yes.

532 MR. CLARKE:

How many?

533 DR. GERDES:

The seven.

534 MR. CLARKE:

Is it then the case that item 52 matched Mr. Simpson at twelve different genetic markers?

535 DR. GERDES:

Again the issue here is the origin of that blood--

536 MR. CLARKE:

Objection, move to strike, nonresponsive.

537 DR. GERDES:

--not the match.

538 THE COURT:

Sustained. Ask the question again.

539 MR. CLARKE:

Dr. Gerdes, isn't it correct that item no. 52, the Bundy blood drop, matched Mr. Simpson at twelve different genetic markers?

KEY QUOTE
540 DR. GERDES:

Yes.

541 MR. CLARKE:

Item no. 56--and first of all, let me return for a moment, if I can, to 47 through 52. Isn't it correct that the PCR-based results for 47, 48, 49, 50 and 52 were all consistent with Mr. Simpson?

542 DR. GERDES:

Yes.

543 MR. CLARKE:

In that series of five blood drops it is also correct that an RFLP match existed to Mr. Simpson, correct?

544 DR. GERDES:

Correct.

545 MR. CLARKE:

Turning to item no. 56, how many genetic markers typed using PCR were consistent with Nicole Brown?

546 DR. GERDES:

Five.

547 MR. CLARKE:

In other words, the five polymarkers, correct?

548 DR. GERDES:

Right.

549 MR. CLARKE:

And you would not include DQ-Alpha because one of the controls didn't operate properly?

550 DR. GERDES:

Correct.

551 MR. CLARKE:

Turning to item no. 78, how many PCR markers were consistent with both Ronald Goldman and Nicole Brown?

552 DR. GERDES:

Well, there is a problem with that particular combination. Okay. I will--D1S80--there is six.

553 MR. CLARKE:

In other words, the five polymarkers and the DQ-Alpha result, those types showed a mixture, correct?

554 DR. GERDES:

Yes.

555 MR. CLARKE:

And that mixture from those six PCR markers; is that right?

556 DR. GERDES:

Yes.

557 MR. CLARKE:

And in fact the results of those six markers showed that neither Nicole Brown nor Ronald Goldman could be excluded, correct?

558 DR. GERDES:

Again when you get to mixtures on the polymarker, you really need to look at those systems that are informative in terms of if you have a combination that picks up both of them, then it includes everybody.

559 MR. CLARKE:

And in fact as to some of the polymarker results in mixtures, that is true, isn't it, where you see each of the combinations possible?

560 DR. GERDES:

Yes.

561 MR. CLARKE:

Turning to--and while we are on 78, as far as the RFLP technology, you have no quarrel with the fact that that particular sample produced a 5-probe RFLP match to both Nicole Brown and Ronald Goldman?

562 DR. GERDES:

That is true.

563 MR. CLARKE:

Would that then constitute eleven different genetic markers as to that sample?

564 DR. GERDES:

Yes.

565 MR. CLARKE:

Turning to the nail clippings, and perhaps we can deal with them all at once, did they produce the same results on each of the three separate items?

566 DR. GERDES:

Yes.

567 MR. CLARKE:

How many different genetic markers produced results consistent with Nicole Brown on each of them?

568 DR. GERDES:

Seven.

569 MR. CLARKE:

And lastly, turning to the rear gate, stains 115, 116 and 117, as to item no. 117, does eight probes sound correct?

570 DR. GERDES:

Yes.

571 MR. CLARKE:

As to the RFLP match with Mr. Simpson?

572 DR. GERDES:

Yes.

573 MR. CLARKE:

How many different PCR markers were also consistent with Mr. Simpson?

574 DR. GERDES:

There is two.

575 MR. CLARKE:

Referring to DQ-Alpha and D1S80?

576 DR. GERDES:

Correct.

577 MR. CLARKE:

Dr. Gerdes, isn't it the case that with respect to the Bundy blood results the RFLP results confirm the PCR markers, whether it is one marker or all seven markers?

578 DR. GERDES:

On that one item, which--it does on the face of it, yes.

579 (Discussion held off the record between the Deputy District Attorneys.)
580 MR. CLARKE:

All right. Your Honor, at this time I would like to use exhibit 260, a similar results board.

581 THE COURT:

All right.

582 (Brief pause.)
583 MR. CLARKE:

Dr. Gerdes, referring you to what is People's exhibit 260, have you again either seen this board or a board with similar results?

584 DR. GERDES:

Yes.

585 MR. CLARKE:

This board contains a number of results obtained using PCR, correct?

586 DR. GERDES:

Yes.

587 THE COURT:

And for the record, this is "Results of DNA analysis of the Bronco."

588 MR. CLARKE:

Thank you, your Honor.

589 MR. CLARKE:

Referring you to the first sample, item no. 23, from the interior of the Bronco, did that produce a result consistent with Mr. Simpson at one genetic marker typed following PCR?

590 DR. GERDES:

Yes.

591 MR. CLARKE:

Does this board in fact show PCR results only from inside the Bronco?

592 DR. GERDES:

Yes.

593 MR. CLARKE:

Referring you to the second item, no. 24, how many genetic markers were typed that were consistent with Mr. Simpson?

594 DR. GERDES:

One.

595 MR. CLARKE:

Referring to 25, how many genetic markers were typed consistent with Mr. Simpson?

596 DR. GERDES:

Two.

597 MR. CLARKE:

Turning to the steering wheel, item no. 29, first of all, how many genetic markers were actually typed following PCR?

598 DR. GERDES:

Six.

599 MR. CLARKE:

And with regard to those samples, is it your opinion also that from a review of those results that neither Mr. Simpson nor Nicole Brown can be excluded?

600 DR. GERDES:

Yes.

601 MR. CLARKE:

Item no. 30, how many PCR genetic markers were used?

602 DR. GERDES:

There are two.

603 MR. CLARKE:

And 31?

604 DR. GERDES:

Two.

605 MR. CLARKE:

From the sidewall, item no. 34, how many?

606 DR. GERDES:

One.

607 MR. CLARKE:

Incidentally, 30--I neglected to ask this. 30 produced a result from two markers consistent with Mr. Simpson?

608 DR. GERDES:

Yes.

609 MR. CLARKE:

31 produced a result at two markers consistent with both Mr. Simpson and Ronald Goldman?

610 DR. GERDES:

Again this is the item where the weak 1.3 is in contention as far as I'm concerned, but that is what they say here, yes.

611 MR. CLARKE:

In other words, you have some criticism of the interpretation of that 1.3 allele, correct?

612 DR. GERDES:

That's correct.

613 MR. CLARKE:

Turning to item no. 34, that result at one marker was consistent with Mr. Simpson?

614 DR. GERDES:

Yes.

615 MR. CLARKE:

What about item 293, the carpet on the driver's side, how many markers produced results that were consistent with Nicole Brown?

616 DR. GERDES:

There are two.

617 MR. CLARKE:

And then lastly, if we can deal with 303, 304 and 305 together, were there in fact two genetic markers that were typed following PCR with those evidence samples?

618 DR. GERDES:

These are the evidence items, this last bunch, that were collected one month later.

619 MR. CLARKE:

Objection, move to strike, nonresponsive.

620 THE COURT:

Sustained. Answer is stricken. The jury is to disregard. Ask the question again.

621 MR. CLARKE:

Dr. Gerdes, items 303, 304 and 305 were typed at how many genetic markers following PCR?

622 DR. GERDES:

Two.

623 MR. CLARKE:

Did they in fact produce results not excluding Mr. Simpson, Ronald Goldman or Nicole Brown?

624 DR. GERDES:

That is what is listed.

625 (Discussion held off the record between the Deputy District Attorneys.)
626 MR. CLARKE:

And then lastly, your Honor, at this time I would like to utilize exhibit 262, a similar results board.

627 (Discussion held off the record between the Deputy District Attorneys.)
628 MR. CLARKE:

Doctor, again, People's exhibit 262, is that a board again similar to one that you have seen either in this form or a different form?

629 DR. GERDES:

Yes.

630 MR. CLARKE:

And with regard to the results starting with sock no. 13, that is the particular item that produced what was a total of a 16-probe match, but was fourteen different probes consistent with Nicole Brown, correct?

631 DR. GERDES:

Correct.

632 MR. CLARKE:

You have conceded or you have offered the opinion that that 14-probe match is in fact a correct one; is that right?

633 DR. GERDES:

Correct.

634 MR. CLARKE:

With regard to that, is that a significant match at fourteen probes?

635 DR. GERDES:

Yes.

636 MR. CLARKE:

And in fact that is--in your opinion would that be an extremely significant match?

637 DR. GERDES:

Again, there is controversy as to how to weigh these things, but in my opinion it is, yes, it would be.

638 MR. CLARKE:

I'm sorry, yes, it would be?

639 DR. GERDES:

Yes.

640 MR. CLARKE:

That sample was typed also at PCR-based markers, wasn't it?

641 DR. GERDES:

Yes.

642 MR. CLARKE:

How many?

643 DR. GERDES:

Two. Well, sorry, seven.

644 MR. CLARKE:

So seven different genetic markers typed following PCR achieved the same results as far as consistency with Nicole Brown as the fourteen RFLP probe matches, correct?

645 DR. GERDES:

Correct.

646 MR. CLARKE:

In your opinion is that an example of an important cross-check between PCR and RFLP results?

647 DR. GERDES:

It can be looked at as a cross-check, yes.

648 MR. CLARKE:

It is a cross-check, isn't it?

649 DR. GERDES:

Yes.

650 MR. CLARKE:

If one of those PCR results produced a result that wasn't consistent with Nicole Brown, wouldn't that potentially raise a question about the reliability of PCR typing in forensics?

651 DR. GERDES:

Yes.

652 MR. CLARKE:

That didn't occur in this case, did it, on this sample?

653 DR. GERDES:

Not on this sample.

654 MR. CLARKE:

Turning to the remainder, and perhaps we can deal with the remaining samples, that is five more evidence items, correct?

655 DR. GERDES:

Yes.

656 MR. CLARKE:

And they were typed at two different genetic markers following PCR?

657 DR. GERDES:

Yes.

658 MR. CLARKE:

They all produced, at least with regard to--we better break it down a little bit.

659 DR. GERDES:

Yes.

660 MR. CLARKE:

42A-2 produced results consistent with Mr. Simpson at two markers, correct?

661 DR. GERDES:

Correct.

662 MR. CLARKE:

42A-3 was actually typed using both PCR and RFLP, correct?

663 DR. GERDES:

Correct.

664 MR. CLARKE:

And in fact the RFLP results revealed an 8-probe match to Mr. Simpson on 42A-3?

665 DR. GERDES:

Yes.

666 MR. CLARKE:

The PCR results were consistent with that RFLP result, weren't they?

667 DR. GERDES:

Yes.

668 MR. CLARKE:

And in fact the PCR results and the RFLP results were the same in being consistent with Mr. Simpson?

669 DR. GERDES:

Yes.

670 MR. CLARKE:

Turning to 42A-4, was that typed at two different PCR genetic markers?

671 DR. GERDES:

Yes.

672 MR. CLARKE:

Did that in fact produce a result consistent with Mr. Simpson?

673 DR. GERDES:

Yes.

674 MR. CLARKE:

And then lastly, turning to 42B-1 and 42B-2, how many genetic markers were typed with those two samples?

675 DR. GERDES:

Two.

676 MR. CLARKE:

Producing results consistent with Nicole Brown, correct?

677 DR. GERDES:

With the exception of 42B-2 where this trace 1.2 needs to be interpreted.

678 MR. CLARKE:

In your view is that possible trace 1.2 the result of real DNA or not?

679 DR. GERDES:

That is the issue here. When you have traces in the system, you can't tell.

680 MR. CLARKE:

Well, I'm asking you with regard to that sample, what is your opinion?

681 DR. GERDES:

In that particular sample I don't know.

682 MR. CLARKE:

All right. Thank you, your Honor.

683 THE COURT:

Are we done with 262?

684 MR. CLARKE:

Yes.

685 (Brief pause.)
686 MR. CLARKE:

May I inquire what time the Court was going to take a break?

687 THE COURT:

10:30.

688 MR. CLARKE:

Very well.

689 MR. CLARKE:

Your Honor, I have one more board I would ask be marked as People's 561--

690 THE COURT:

571.

691 MR. CLARKE:

571.

692 (Peo's 571 for id = posterboard)
693 (Discussion held off the record between the Deputy District Attorneys.)
694 MR. CLARKE:

Your Honor, for the record this board could be described as a board labeled "Concordance of Cellmark results with DOJ results."

695 THE COURT:

Yes.

696 MR. CLARKE:

Dr. Gerdes, with respect to, and let's start with the first item, 13A-1, is it correct, and we have discussed this to some extent a few moments ago, that the RFLP results obtained by each laboratory were consistent with one another, correct?

697 DR. GERDES:

Correct.

698 MR. CLARKE:

And that is with the fact that as to that sample there were a number of RFLP probes that matched Nicole Brown, correct?

699 DR. GERDES:

Correct.

700 MR. CLARKE:

Is that an example of, as you have used the term, significant match?

701 DR. GERDES:

Yes.

702 MR. CLARKE:

There were also PCR results that you described a few moments ago that were also consistent with Nicole Brown from each laboratory, correct?

703 DR. GERDES:

On that item, yes.

704 MR. CLARKE:

And in fact Cellmark's DQ-Alpha and polymarker results were consistent with the Department of Justice's DQ-Alpha and D1S80 results, correct?

705 DR. GERDES:

Correct.

706 MR. CLARKE:

Would this be an example where different laboratories achieve the same results from the same sample?

707 DR. GERDES:

Yes.

708 MR. CLARKE:

Turning to the steering wheel, that particular item was typed by Cellmark at six genetic markers and by DOJ at one of the same markers, DQ-Alpha, correct?

709 DR. GERDES:

Correct.

710 MR. CLARKE:

Those results were consistent with one another, weren't they?

711 DR. GERDES:

Yes.

712 MR. CLARKE:

Turning to item 47 on the Bundy walkway, Cellmark's six genetic marker results were consistent with DOJ's two genetic marker results, one of which was typed by both laboratories, one marker?

713 DR. GERDES:

Yes.

714 MR. CLARKE:

Turning to item no. 48 from the Bundy walkway, is it correct that the six genetic markers typed by Cellmark were consistent with the two genetic markers typed by the Department of Justice, again one of which was the same DQ-Alpha marker?

715 DR. GERDES:

Yes.

716 MR. CLARKE:

In these instances, 47, 48, in fact two laboratories tested material from the same sample using the same genetic marker, didn't they?

717 DR. GERDES:

Yes.

718 MR. CLARKE:

Doesn't that constitute a cross-check of the accuracy of typing results by a laboratory?

719 DR. GERDES:

Typing that item, yes.

720 MR. CLARKE:

Turning to item 50, again six genetic markers were typed at Cellmark and two genetic markers at DOJ, one of which was the same DQ-Alpha marker?

721 DR. GERDES:

Yes.

722 MR. CLARKE:

And in this series, item 52 produced RFLP results obtained by Cellmark, correct?

723 DR. GERDES:

Yes.

724 MR. CLARKE:

And six additional genetic markers following PCR at Cellmark?

725 DR. GERDES:

Yes.

726 MR. CLARKE:

And then again two PCR-based markers were typed at the Department of Justice obtaining results the same as at Cellmark, correct?

727 DR. GERDES:

For that DNA, yes.

728 MR. CLARKE:

And then lastly, turning to item no. 84, and that was a series of three different samples from fingernail clippings and scrapings, correct?

729 DR. GERDES:

Correct.

730 MR. CLARKE:

Actually had three different numbers because there were three different items?

731 DR. GERDES:

Uh-huh.

732 MR. CLARKE:

With regard to that sample, Cellmark typed them at six genetic markers?

733 DR. GERDES:

Yes.

734 MR. CLARKE:

Consistent with Nicole Simpson?

735 DR. GERDES:

Yes.

736 MR. CLARKE:

Nicole Brown Simpson?

737 DR. GERDES:

Yes.

738 MR. CLARKE:

And DOJ typed those samples at one genetic marker, correct?

739 DR. GERDES:

Correct.

740 MR. CLARKE:

As far as the results from those two different laboratories, they are in fact consistent with one another in terms of the fact that Nicole Brown could not be excluded as the donor of that DNA, correct?

741 DR. GERDES:

That's correct.

742 MR. CLARKE:

Isn't this inter-laboratory comparison, in fact, when one looks at the number of samples, a cross-check of the accuracy of results?

743 DR. GERDES:

It is a cross-check of the items that were extracted by those labs, yes.

744 MR. CLARKE:

All right. Your Honor, I was going to change into another area that was going to take--actually be my final area, but it will take longer than five minutes.

745 THE COURT:

Well, let's use the five minutes we have.

746 MR. CLARKE:

Very well.

747 (Brief pause.)
748 MR. CLARKE:

Dr. Gerdes, this case doesn't involve testing of one or two evidence items, such as one or two bloodstains on a sidewalk, does it?

749 DR. GERDES:

That's true.

750 MR. CLARKE:

In fact, in this case there are more than forty different evidence samples that produced DNA typing results; isn't that correct?

751 DR. GERDES:

That sounds about right.

752 MR. CLARKE:

Isn't it correct that with respect to the negative controls in this case, unstained substrate controls, that is one negative control, correct?

753 DR. GERDES:

Correct.

754 MR. CLARKE:

A reagent blank, is that another negative control for PCR typing?

755 DR. GERDES:

Correct.

756 MR. CLARKE:

There is also a negative amplification control, as we discussed last Friday or Thursday?

757 DR. GERDES:

Correct.

758 MR. CLARKE:

Isn't it correct that all of those controls, the substrate controls, the reagent blank, the negative amplification controls in this case show no evidence of contamination?

759 DR. GERDES:

There is one item from Cellmark that is a reagent blank that shows some evidence of contamination. With that one exception all the rest appear clean.

760 MR. CLARKE:

How many different runs did these approximately forty or more than forty evidence items include in this case? I'm referring to PCR results?

761 DR. GERDES:

Well, I haven't counted them.

762 MR. CLARKE:

Well, would samples be run more than once?

763 DR. GERDES:

Yes.

764 MR. CLARKE:

How many times--

765 DR. GERDES:

Some samples; other samples not.

766 MR. CLARKE:

So samples would be run one time?

767 DR. GERDES:

Yes.

768 MR. CLARKE:

What is the most another sample--I'm referring to evidence material. What is the most times another sample would be run?

769 MR. SCHECK:

Your Honor, I think this is vague and repetitive.

770 THE COURT:

Sustained. It is vague.

771 MR. CLARKE:

In terms of the use of the term "Run," how do you use that term?

772 DR. GERDES:

Well, in terms of the table that I formulated, it basically just refers to a specific date on which a series of strips were tested.

773 MR. CLARKE:

Okay. Were the same types of steps undertaken with regard to the evidence in this case?

774 DR. GERDES:

Yes.

775 MR. CLARKE:

So can we use the word "Run" to describe this different date that a sample might be typed in this case?

776 DR. GERDES:

That is--yes.

777 MR. CLARKE:

That would include amplification through actually determining which dots or which types are present?

778 DR. GERDES:

Yes.

779 MR. CLARKE:

With regard to these more than or approximately forty evidence items, how many different runs would those include in--you described that one sample might be run only once. How many times might another sample be run?

780 DR. GERDES:

I would have to look at a specific item and count these things. I haven't done that.

781 MR. CLARKE:

Okay. Is it correct that a sample might be run more than once?

782 DR. GERDES:

Some samples were, yes.

783 MR. CLARKE:

So is it the case that in this case there are more than forty different runs?

784 DR. GERDES:

Again I would have to count them up. I haven't done that.

785 MR. CLARKE:

Many of these samples were run by three different laboratories, correct?

786 MR. SCHECK:

Your Honor, this is asked and answered.

787 THE COURT:

We have already gone through that.

788 MR. CLARKE:

As far as the number of runs, wouldn't it be much more than forty?

789 MR. SCHECK:

Same objection.

790 THE COURT:

Overruled.

791 DR. GERDES:

If you count the runs in all the different laboratories, again, I--it is perhaps that many; perhaps not. I haven't counted them up, but there were a number of them.

792 MR. CLARKE:

Could it be as many as eighty to a hundred different runs?

793 DR. GERDES:

I doubt that.

794 MR. CLARKE:

If you had to place an estimate on it, what estimate would you place?

795 DR. GERDES:

Let me take a quick look here.

796 (Brief pause.)
797 THE COURT:

All right. Mr. Clarke, Dr. Gerdes, why don't we take our break at this point. You can take a look and see what your records say. I don't want to rush you while you are on the stand here. Ladies and gentlemen, we are going to take our mid-morning break. Please remember all my admonitions to you. We will stand in recess for fifteen minutes. All right.

Temperature

tense

Key Quotes (5)

Dr. John Gerdes
It says 'Lam flow.' Well, at that time I hadn't really noticed that this wasn't a laminar flow hood. I put down 'Laminar flow' and I didn't really realize until the second visit that this wasn't a laminar flow hood.
Clarke catches Gerdes contradicting his own trial testimony using Gerdes's own handwritten notes from his December 1994 lab tour — he had testified LAPD lacked a laminar flow hood, but his notes labeled the hood as 'Lam flow.'
Dr. John Gerdes
Most of our testing could be described as home-brewed as opposed to commercial kits.
Clarke reads from Gerdes's own federal grant proposal, contradicting his trial testimony that his lab used mostly FDA-approved commercial kits — forcing Gerdes into a lengthy explanation about R&D versus routine testing.
Dr. John Gerdes
I probably said that, but you have to look at the context around that... When you said it, did you mean it? Yes.
Gerdes concedes he previously testified that cross-checking PCR and RFLP results on enough samples is 'a significant method of validation' — directly undermining the blanket anti-PCR position he took on direct examination.
Dr. John Gerdes
Yes... Yes... Yes... That's correct... No.
In a long series of questions about RFLP probe matches (5, 6, 8, 14 probes), Gerdes is forced to concede that virtually all RFLP results in the case — on the glove, socks, Rockingham foyer, Bundy walkway, and rear gate — are accurate and match the prosecution's theory.
George Clarke
Dr. Gerdes, isn't it correct that item no. 52, the Bundy blood drop, matched Mr. Simpson at twelve different genetic markers?
After Gerdes tries to pivot to contamination, Clarke forces a direct yes/no answer — Gerdes concedes 12 genetic markers match Simpson on item 52, the drop Gerdes had claimed was contaminated.

Evidence (6)

People's 1301
Photograph of the LAPD DNA laboratory showing the hood
displayed to witness to establish laminar flow hood existed
People's 569
Xerox copy of Gerdes's handwritten notes and rough diagram from his December 1994 LAPD lab tour, showing 'Lam flow' notation next to the hood
introduced and displayed; used to impeach Gerdes's testimony that LAPD lacked a laminar flow hood
People's 570
Posterboard titled 'Results of RFLP DNA Analysis' listing samples from the glove, Rockingham foyer, socks, Bundy walkway, and rear gate with probe counts and non-excluded individuals
introduced; Gerdes steps down from stand to review; used to extract concessions on RFLP accuracy
People's 259
Bundy results board showing PCR genetic marker results for items 47–78 and nail clippings
displayed; Clarke walks Gerdes through each item extracting concessions on marker counts consistent with Simpson, Brown, and Goldman
People's 260
Results board titled 'Results of DNA Analysis of the Bronco' showing PCR results from interior Bronco samples
displayed; Clarke begins walking through Bronco items (transcript truncated)
Informal
Gerdes's federal grant proposal, page 31, containing the phrase 'most of our testing could be described as home-brewed as opposed to commercial kits'
quoted by Clarke to impeach Gerdes's testimony about kit usage

Notable Exchanges (4)

George ClarkeDr. John Gerdes
Clarke reads Gerdes's own grant proposal language describing lab methods as 'home-brewed' after Gerdes testified that most of his lab's testing used FDA-approved commercial kits. Gerdes tries to distinguish R&D from routine testing but acknowledges the statement.
strategic
George ClarkeDr. John GerdesBarry Scheck
Clarke attempts to impeach Gerdes with prior testimony from the McIntosh case about RFLP/PCR cross-validation, but lacks the specific transcript page. Scheck moves to strike; Ito overrules but warns Clarke it is subject to a motion to strike. Clarke eventually drops the specific quote.
procedural friction
George ClarkeDr. John Gerdes
Clarke extracts that the sock stains matched Nicole Brown at 14 RFLP probes across DOJ and Cellmark (11 + 5, minus 2 shared), and that Gerdes has no quarrel with that result. Gerdes confirms the arithmetic himself.
revealing
George ClarkeDr. John Gerdes
On item 52 (Bundy blood drop), Gerdes attempts to qualify his concession of a 12-marker match by pivoting to contamination as the origin of the DNA. Clarke objects as nonresponsive; Ito sustains and directs Gerdes to answer the question, ultimately forcing a clean 'Yes.'
heated

Credibility Attacks (4)

⚔ Dr. John Gerdes
prior inconsistent statement (own notes)
Clarke used Gerdes's own handwritten diagram from his December 1994 LAPD lab visit, which labeled the hood 'Lam flow,' to contradict his trial testimony that LAPD's lab lacked a laminar flow hood. Gerdes conceded the note was written incorrectly.
⚔ Dr. John Gerdes
prior inconsistent statement (grant proposal)
Clarke quoted Gerdes's federal grant proposal stating 'most of our testing could be described as home-brewed,' contradicting Gerdes's testimony that his lab primarily used FDA-approved commercial kits. Gerdes acknowledged writing the statement.
⚔ Dr. John Gerdes
prior inconsistent testimony (McIntosh case)
Clarke impeached Gerdes with testimony from the McIntosh case (under a year prior) in which Gerdes stated that cross-checking PCR and RFLP results on enough samples is 'a significant method of validation' — directly contradicting his blanket opposition to PCR in this case. Gerdes acknowledged he had said it and meant it at the time.
⚔ Dr. John Gerdes
concession through systematic questioning
Clarke walked Gerdes through every major RFLP result in the case — glove, socks, Rockingham, Bundy, rear gate — extracting admissions that each result was accurate and that the RFLP data confirmed the PCR results, effectively neutralizing Gerdes's core trial thesis.

Witness Demeanor

(Witness steps down from stand to review RFLP board)
(Brief pauses for off-record discussions between DAs before several questions)
(Witness checks notes during testimony to verify specific DOJ sequencing dates)

Objections

14 objections (5 sustained, 7 overruled)
Proceeding 7195 • 797 utterances • Defense witness
Criminal Trial
Department 103
⚖️ Start
📂 AUG 7, 1995 📄 Cross-examination of Dr. John
AUG 7, 1995 KRT DvH TD