George Clarke cross-examines defense DNA expert Dr. John Gerdes on his contamination findings from LAPD crime lab validation studies, methodically getting Gerdes to concede that the unexpected alleles he flagged as contamination could alternatively be explained by DX gene activity or cross-hybridization — neither of which would constitute contamination. The proceeding is largely technical and procedural, briefly interrupted when the courtroom's projection monitor fails, forcing Clarke to revert to marking physical exhibits by hand and passing them to the jury.
# 1 MR. CLARKE: All right. With the Court's permission I would like to use the first People's exhibit 566.
# 3 MR. CLARKE: Now, just while we are at the board--doctor, would it be easier for you at the witness stand or would it be easier for you to come down to see what is projected?
# 4 DR. GERDES: I believe if I can operate from my photo, if I have to be back to the table to look at the recording, it would be best from here for the moment.
# 5 MR. CLARKE: That is fine. Just in terms of People's exhibit 566, this is hybridization record 182?
# 6 THE COURT: Can't read it.
# 7 MR. CLARKE: On the upper right-hand corner. Actually zoom in on the very top of the document.
# 8 THE COURT: How about the right-hand?
# 9 DR. GERDES: Over to the right-hand side. Yes, this is 182.
# 10 MR. CLARKE: And this is a series of eight strips that were run on May 6th, 1994?
# 12 MR. CLARKE: And is this one of the, as we have used the term, validation studies that you looked at as part of your review?
# 13 DR. GERDES: These are some of the samples I have looked at, yes.
# 14 MR. CLARKE: The analyst in this case, or at least in the instance of these eight samples, was Collin Yamauchi?
# 16 MR. CLARKE: And the confirming analyst was Erin Riley?
# 17 DR. GERDES: That's correct.
# 18 MR. CLARKE: Is it correct that none of these were case work samples?
# 19 DR. GERDES: That's correct.
# 20 MR. CLARKE: You identified in this particular set of eight samples two samples as containing contamination; is that right?
# 21 DR. GERDES: That's correct.
# 22 MR. CLARKE: Would the--
# 23 DR. GERDES: Basically they were identified as contamination and/or DX.
# 24 MR. CLARKE: And that was, in your opinion, the result, that is, these reactions, of either contamination or this DX gene activity?
# 25 DR. GERDES: In these specific instances again these are 1 dot, they contain a 1 dot, so this is one of those cases where you can't tell.
KEY QUOTE # 26 MR. CLARKE: Could you tell us, in terms of the photographs that go from top to bottom, and let's start with the first one, which would be easier to start with, from your records?
# 27 DR. GERDES: In terms of which one was identified as a contaminant?
# 29 DR. GERDES: Let's start with no. 2.
# 30 MR. CLARKE: Okay. That would be the sample and perhaps we can zoom in on that horizontal strip. In fact, I don't know if you can tell, doctor, but in terms of talking about both samples, is this zooming point going to be enough to be able to describe both samples that you have offered opinions about?
# 32 MR. CLARKE: All right. Now, the second sample down is labeled off to the far right "52-2"?
# 34 MR. CLARKE: And the analyst noted a particular result with that sample; is that right?
# 35 DR. GERDES: That's correct.
# 36 MR. CLARKE: What did the analyst note?
# 37 DR. GERDES: A 1.2, 1.2 and then there is a notation that is difficult to read, but it says "With 1.1 very faint."
# 38 MR. CLARKE: Okay. That is actually written by the analyst off to the far right?
# 40 MR. CLARKE: Perhaps if we could just show that off to the right of that sample, I'm sorry, by going down in that the written portion.
# 41 MR. CLARKE: The results are actually written in; isn't that right, doctor?
# 42 DR. GERDES: That's correct.
# 43 THE COURT: I believe to the right.
# 44 DR. GERDES: You have to go to the right.
# 45 MR. CLARKE: And is that, Dr. Gerdes, when you look at the second row down, where you have described 1.2, 1.2, and then is that with--I'm sorry, weak 1.1?
# 46 DR. GERDES: That's correct. That is what that says.
KEY QUOTE # 47 MR. CLARKE: Okay. And in your opinion and what you have described in your chart and the summary of your findings that that 1.1 you have offered the opinion is either a contaminant or this DX activity?
# 48 DR. GERDES: That's correct. You can see it on the photograph. It was recorded by the analyst. It is an additional allele compared to what the analyst has recorded.
KEY QUOTE # 49 MR. CLARKE: And in fact--
# 50 DR. GERDES: And therefore represents, you know, this--a--either DX or a contaminant.
# 51 MR. CLARKE: And this is because you know the sample's type is a 1.2, 1.2, right? It is from a known person?
# 52 DR. GERDES: That's correct, proficiency testing, and that was the correct type that it should be.
# 53 MR. CLARKE: All right. If we could then zoom in on the strip as we had it previously. And what I'm going to ask you to do when we reach that point, Dr. Gerdes, and perhaps we can use the arrows to actually locate the arrow next to the dot on the sample, this second sample down, where you concluded that there was an additional 1.1 contaminant or DX gene activity?
# 54 DR. GERDES: Do you want me to do that?
# 55 MR. CLARKE: Well, would it--let's see if we can just place it while we are there.
# 57 MR. CLARKE: If we take the arrow and go to the left or go off the screen.
# 59 DR. GERDES: It is on the monitor here still?
# 60 THE COURT: I think we just lost our projection. We may have to do this the old-fashioned way.
# 61 MR. CLARKE: Or ask jurors to crowd around the monitor, but I don't think that will work.
# 62 THE COURT: It appears to me that we have lost our large monitor unit. Matt?
# 63 MR. ORMOND: Yes, sorry.
# 64 THE COURT: How long does it take us to switch in the back-up?
# 65 MR. ORMOND: Ten to fifteen minutes.
# 66 THE COURT: Mr. Clarke, what is your pleasure?
# 67 MR. CLARKE: I would prefer to continue on based on the foundation I have already laid, rather than interrupt this topic.
# 68 THE COURT: All right. Proceed. All right. I do have a monitor here in front of juror no. 1 that is still working; is that correct?
# 70 THE COURT: Matt, how long is the cable on the monitor in front of monitor no. 1?
# 71 MR. ORMOND: About 50 feet. I can move it.
# 72 THE COURT: It is a large monitor. It is 20-inch monitor; is that correct?
# 73 MR. ORMOND: 21 inches.
# 75 MR. CLARKE: May I make a suggestion?
# 76 MR. SCHECK: Can we move this one?
# 77 MR. CLARKE: I will proceed if I can do it the old-fashioned way and have the witness place an arrow on the exhibit and then pass it to the jury.
# 78 THE COURT: Be my guest.
# 79 MR. CLARKE: All right. I am certainly used to doing it that way.
# 80 THE COURT: That is the way they do it in Oklahoma, I'm sure.
KEY QUOTE # 81 MR. CLARKE: May I approach the witness, your Honor?
# 83 MR. CLARKE: All right. Dr. Gerdes, on People's exhibit 566, if you would--and do you need a--perhaps we can use a fine pen.
# 84 DR. GERDES: Would have a fine pen there?
# 85 MR. CLARKE: I have a felt pen.
# 86 DR. GERDES: Regular pen. Regular pen is okay.
# 87 MR. CLARKE: With regard to that 1.1 allele on that second sample down--and in fact is that second sample labeled "52-2"?
# 88 DR. GERDES: Yes, it is.
# 89 MR. CLARKE: Okay. Could you place an arrow, and if it is possible to do that without interrupting any other of the results, to point out the 1.1 dot where it is located on that sample. And would a circle around it be easier?
# 90 DR. GERDES: That would be fine.
# 93 MR. CLARKE: Okay. You have placed that circle--
# 95 MR. CLARKE: Does that circle then now basically surround the dot where you detected to be either a contaminant or DX activity?
# 96 DR. GERDES: I have circled both the number that designates it and just to the right of that number is where this dot--very faint dot is located.
# 97 MR. CLARKE: First of all, that dot is clearly less than the C dot, correct?
# 99 MR. CLARKE: And the C dot is important in the correct interpretation of sample, correct?
# 100 DR. GERDES: It is important in the correct interpretation of samples that are known not to be mixtures. Once you have any suspicion of a mixture it is no longer used for that function.
# 101 MR. CLARKE: Well, doesn't the user guide say the C dot is to be used as a guide in interpreting the presence of typeable alleles?
# 102 DR. GERDES: Yes. That is what I explained earlier. If the C dot is not present, that is why this is important. If it is not there you have too little DNA. If it is there and you have any indication of more than the number of alleles you should have, then it is no longer of any function.
# 103 MR. CLARKE: While you have that photograph, was there a second sample that you believed similarly contained a contaminant or DX activity?
# 104 DR. GERDES: Yes, there is.
# 105 MR. CLARKE: Would that be how far down?
# 106 DR. GERDES: Two strips down from the one we just discussed or 52-4.
# 107 MR. CLARKE: Could you circle that particular 1.1 dot?
# 108 DR. GERDES: Certainly.
# 109 MR. CLARKE: In the same manner as the previous one?
# 110 (Witness complies.) # 111 MR. CLARKE: And you have done that?
# 112 DR. GERDES: I have.
# 113 MR. CLARKE: For purposes of your chart, those two samples as a result of that 1.1 activity were listed as unexpected alleles, correct?
# 114 DR. GERDES: That's correct, because the--these are proficiency samples. The typing--
# 115 MR. CLARKE: I'm sorry, objection, nonresponsive, move to strike.
# 117 THE COURT: Overruled.
# 118 DR. GERDES: They are proficiency samples. The--the typings--expected typings are known, they are not mixtures, they are not supposed to be mixtures as they were provided, and this is an additional allele over those anticipated type results.
# 119 THE COURT: Next question.
# 120 MR. CLARKE: Was it listed in your chart?
# 121 DR. GERDES: It was.
# 122 MR. CLARKE: If that 1.1 reaction was as a result of this DX gene, it wouldn't be a contaminant, correct?
# 123 DR. GERDES: In this case you can't tell if it is a DX or not.
# 124 MR. CLARKE: Objection, nonresponsive.
# 125 THE COURT: Sustained. That is not the question, doctor.
# 126 MR. CLARKE: Doctor, if that activity in the 1.1 dot was from the DX gene, it wouldn't be a contaminant, would it?
KEY QUOTE # 127 DR. GERDES: That's correct.
# 128 MR. CLARKE: If that 1.1 dot was the result of cross-hybridization because of the presence of the 1.2 allele, then that activity would not be from a contaminant, correct?
# 129 DR. GERDES: Cross-hybridization is not well-known for this particular gene other than the DX phenomena, but that is true.
# 130 MR. CLARKE: So it in fact would be true that it would not be from a contaminant if it was because of cross-hybridization with the 1.2 allele?
# 131 DR. GERDES: There is another explanation for this particular allele.
# 132 MR. CLARKE: And in fact this cross-hybridization is described in the user guide, correct?
# 133 DR. GERDES: It is described in the presence of high levels of DNA, yes.
# 134 MR. CLARKE: It is described in the package insert, isn't it?
# 135 DR. GERDES: Yes, it is.
# 136 MR. CLARKE: And it is described in the scientific literature about DQ-Alpha typing?
# 138 MR. CLARKE: All right. Your Honor, at this time I would ask that the photograph be passed to the jury.
# 139 THE COURT: All right. Let's hand it to juror no. 7.
# 140 (The exhibit was passed amongst the jurors.) # 141 THE COURT: All right. Mr. Clarke, would you collect that from Deputy Long, please.
# 142 MR. CLARKE: Yes, thank you.
# 143 THE COURT: Let the record reflect that all the juror members have had an opportunity to review the exhibit. Mr. Clarke.
# 145 MR. CLARKE: Dr. Gerdes, now, showing you what I believe has been marked People's exhibit 567, I believe you stated already that that appears to be another similar photograph of what was two hybridization records later than the previous run?
# 147 MR. CLARKE: Was that part of the same DQ-Alpha run by Collin Yamauchi?
# 148 DR. GERDES: Yes, same date.
# 149 MR. CLARKE: And in this particular instance you identified two more samples that you believed demonstrated a contaminant?
# 150 DR. GERDES: Yes, 52-20 and 52-22, which is the fourth strip down and the sixth strip down.
# 151 MR. CLARKE: Okay. You have used the identifier 52-20 to describe it?
# 152 DR. GERDES: Yeah, because you could use lane 4 and lane 6.
# 153 MR. CLARKE: Okay. You concluded from that run--and again this is a series of non-case work samples?
# 154 DR. GERDES: Yes, that's correct, they are all standards or proficiency.
# 155 MR. CLARKE: Let's start with 52-20. The actual type called by the analyst is a 1.2, 2?
# 157 MR. CLARKE: Do you agree with that typing?
# 159 MR. CLARKE: That was a correct typing?
# 161 MR. CLARKE: You also, as a result of your review of all of these strips, concluded that in your opinion there were two other alleles present that you felt were contamination?
# 162 DR. GERDES: On that particular strip, yes.
# 163 MR. CLARKE: And what alleles were those?
# 164 DR. GERDES: The 1.1 and the 1.3 are--are visible on that strip.
# 165 MR. CLARKE: Could you then, with the pen, circle those two alleles, the 1.1 and the 1.3 that you felt were contaminants, as you did with the previous photo?
# 166 DR. GERDES: On 52-20?
# 168 DR. GERDES: Okay. (Witness complies.)
# 169 MR. CLARKE: And did you do that?
# 171 MR. CLARKE: Thank you. Also on that same run you identified another sample as containing contaminating alleles; is that correct?
# 172 DR. GERDES: That's correct.
# 173 MR. CLARKE: That was which sample?
# 174 DR. GERDES: Well, lane--hold on. That would be lane 6.
# 175 MR. CLARKE: And does it have a 52 dash number, just for the record?
# 177 MR. CLARKE: What alleles were called by the analyst?
# 178 DR. GERDES: A 1.1, 3 with a very weak 1.2, 1.3, 4 and a 1.3.
# 179 MR. CLARKE: Okay. First of all, the type that is called by the analyst--and I may have misunderstood you--did you say a 1.1?
# 180 DR. GERDES: 1.1, 3.
# 181 MR. CLARKE: And then you also described the analyst made some notations about some other dots?
# 182 DR. GERDES: Yes. It is noted very weak 1.2, 1.3, 4, that is that dot that detects all of those, and a 1.3.
# 183 MR. CLARKE: Okay. Just to be clear, when the analyst wrote down "Very weak 1.2, 3 and 4," the analyst wasn't noting that the analyst saw a very weak 1.2, a very weak 3 and a very weak 4?
# 184 DR. GERDES: That is 1 dot. That is that dot that you see all three of those on that 1 dot.
# 185 MR. CLARKE: So the analyst is really noting that is a dot that I saw a very weak pattern?
# 186 DR. GERDES: Correct.
# 187 MR. CLARKE: Not that I saw a very weak.
# 188 MR. CLARKE: Those three individual alleles?
# 189 DR. GERDES: That's correct.
# 190 MR. CLARKE: Did the analyst make any other notation?
# 191 DR. GERDES: No. Well, the 1.3 which I discussed earlier, yes.
# 192 MR. CLARKE: As far as your review and the opinions that you offered as a result of reviewing that strip, did you then conclude that they were contaminating alleles?
# 193 DR. GERDES: Yes. This is a standard. It should only have the two types and it has these additional dots.
# 194 MR. CLARKE: And the additional alleles were what?
# 195 DR. GERDES: They would be consistent with a 1.2 and a 1.3.
# 196 MR. CLARKE: Now, these alleles, 1.2 and 1.3, were clearly less than the C dot, correct?
# 197 DR. GERDES: Yes, they are.
# 198 MR. CLARKE: As far as the call on the sample by the analyst, do you agree with the call of the actual typing results?
# 200 MR. CLARKE: The typing results of 1.1 and 3 were called correctly by the analyst; is that right?
# 201 DR. GERDES: That's true.
# 202 MR. CLARKE: With regard to--and you described the fact that this sample, the person actually has the 1.1 allele, correct?
# 204 MR. CLARKE: If the reaction in the 1.2--and again, the 1.2 is a very close probe to the 1.1, right?
# 205 DR. GERDES: There is a single-base pair difference.
# 206 MR. CLARKE: If the 1.2 reaction were as a result of cross-hybridization it would not be a contaminant, correct?
# 207 DR. GERDES: That phenomenon is not reported to be a problem with that particular dot, but--theoretically, yes.
# 208 MR. CLARKE: Objection, nonresponsive, your Honor.
# 209 THE COURT: Overruled.
# 210 MR. CLARKE: You said the answer is yes?
# 211 DR. GERDES: Yes, but it has not been reported to be a problem on that dot.
# 212 MR. CLARKE: When you say it is not reported to be a problem, what do you mean?
# 213 DR. GERDES: In laboratories, the number of laboratories I have looked at and in the literature, there is no report that the 1.2 cross-hybridizes.
# 214 MR. CLARKE: Dr. Gerdes, isn't it true the scientific literature does describe cross-hybridization of all of the 1 subtypes; 1.1, 1.2 and 1.3?
# 215 DR. GERDES: As far as what is observed in laboratories the 1.1 and the 1.3 are observed--
# 216 MR. CLARKE: Objection, nonresponsive, your Honor.
# 217 THE COURT: Sustained.
# 218 MR. CLARKE: Dr. Gerdes, doesn't the scientific literature describe cross-hybridization between all three of the 1 subtypes; 1.1, 1.2 and 1.3?
# 219 DR. GERDES: I don't believe so.
# 220 MR. CLARKE: If the 1.3 allele--and we discussed just the 1.2 at this point, correct?
# 221 DR. GERDES: That's correct.
# 222 MR. CLARKE: If the 1.3 allele were from cross-hybridization, it would not be a contaminant, correct?
# 223 DR. GERDES: That's true.
# 224 MR. CLARKE: All right. Your Honor, then with the Court's permission I would like this particular photograph be passed to the jury.
# 225 THE COURT: All right.
# 226 DR. GERDES: Would you like me to mark those dots?
# 227 MR. CLARKE: Oh, I'm sorry. Yes, sir. Please, doctor.
# 228 DR. GERDES: Okay. (Witness complies.)
# 229 THE COURT: All right. Mr. Clarke, would you hand that to juror no. 7, please.
# 230 (The exhibit was passed amongst the jurors.) # 231 MR. CLARKE: May we approach for a brief moment, your Honor? This relates to logistics.