(BY MR. P. BAKER) You told this jury you visited the lab basically to give an unbiased opinion to the standards, right?
I would say he's absolutely not a friend. I mean I knew him in no way, shape or form before I became involved in this case.
And your opinion is that everything was hunky dory in terms of the examination of the swatches?
It's generally held in the scientific community that approximately a thousand nanograms are found in a drop of blood, right?
I would say that's probably in the right neighborhood. Then again, I wouldn't testify to any of the numbers if I don't look. It's a specific science.
A question is not pending, sir. How many nanograms were found in Item 48, about five, more or less?
Seems to me that the lawyer is testifying here other than the witness.
MR. P. BAKER: Your Honor, he's testifying as to the DNA in this case. If he doesn't know the nanograms as to the Bundy walkway, we got problems.
(BY MR. P. BAKER) I just have my notes. I don't have the exact drawings, you understand, of the five Bundy swatches. The maximum amount of nanograms is 33.6, right?
Again, I'd have to look at the bench notes for that. I mean I'll -- basically, if you put a number up there and you want me to give you an interpretation of what it means, I'll be happy to do that.
Let's assume for a hypothetical Item 52 had 31.6 nanograms, Item 58 had 12.1, Item 49 had 1.8, Item 48 only had 5?
I mean I -- again, that part of the transcript -- I mean I may have read that at one point. Again I -- again, that's not something that was fundamental to my part of this investigation.
Okay. Now, you can have contamination in terms of DNA from simply changing gloves, right, you can transfer DNA by changing gloves, transfer from one to the other, right?
Sure. If you have DNA on your right hand and you touch the left hand with it, you can transfer the DNA?
Now, did the plaintiffs show you the demonstration that Andrea Mazzola did of her best collection techniques?
They never showed you -- they never showed you the videotape that Andrea Mazzola presented at the prosecution of -- about what was her best collection techniques?
Okay. You never heard that in that videotape she touches the pavement, moves the swatches, transfers the tweezers, touches her glasses; you never heard anything like that?
I mean I have heard bits and pieces of this, yes -- I mean from the transcript, reading the transcript of the civil trial.
And you have no independent knowledge, as you sit here today, how Andrea Mazzola collected the swatches at the crime scene, correct?
That's another liquid that's used when you're working with DNA; the liquid you use in the process, correct?
Well, there were some reagents that were that old, but I saw no reason to worry about those, no, I didn't.
So you had no concerns about reagent contamination at the LAPD crime lab; is that your testimony?
I see absolutely no evidence of reagent contamination at LAPD. That is my testimony.
KEY QUOTEIn terms of the work on this case, I -- the work presented in this case, I have seen no evidence of reagent contamination.
I'm talking about the civil case and the criminal case. You've never said to anyone that you were concerned about reagent contamination at the LAPD crime lab?
I mean something has to make that transfer occur. But it can occur -- you can't see DNA, so sure, it can occur without you seeing it.
And you're talking about Mr. Yamauchi's work with the reference vial, and you basically told this jury you had no criticism of Colin, right?
I have no criticism whatsoever of the way he removed the blood from that vial, that's correct.
Now, Dr. Popovich, you wouldn't work at the same area with the reference vial and evidence samples, right? You wouldn't?
Well, if one is working with a sample with high DNA amounts and other samples with lower DNA amounts, one can work with those very easily at the same time, yes.
Well, one has to be conscious, one has to -- one has to know what they're doing to do that, but yes, one can do it, and we do that in my own lab.
I'm going to read page 54 of your deposition taken on August 15, 1996. (Mr. P. Baker read a portion of the deposition transcript of Bradley Popovich.)
Is it good practice to process reference samples at the same location within the same sitting as evidence samples?
Well, it all comes down -- I mean one has to be very precise about this. I mean if you're asking me is it good practice for me to have a reference tube of blood and right next to it have evidence that I'm working with that is going to be in trace amounts, potentially in trace amounts, the answer is no, that's not good practice.
You would work with the reference vial on the same table as evidence samples, is that what you're telling this jury?
Misstates the evidence if we're talking about what Colin Yamauchi did.
MR. P. BAKER: I'm asking a question.
We work with samples with high concentration and low concentration samples on the same table all the time.
It entirely depends on the sensitivity of what one's trying to do. If one is executing a procedure where one is looking for single molecules of DNA, it would be absolutely essential to do -- to bleach, to change the paper, to do everything that you're alluding to. If one's working with a test that has a much higher threshold of sensitivity, it becomes necessary to do those types of things. It all depends on what you're threshold of sensitivity is.
The LAPD didn't bleach down the area, change the paper, or use UV rays on the notes when they processed these evidence samples, right?
It's my understanding from personal conversations with Colin Yamauchi, and from his transcript, that he did not work with a reference vial of blood at the same place that he worked with the samples on June 14.
Well, it's my understanding from being in the room and having him show me exactly where he did it.
Well, he took me in the room and said, on June 14 I sat here and did this and I went over there and did that.
If he testified differently, your opinion as to how he handled the reference samples would be null and void?
It's like saying if he said something different than what he said, his opinion would be different. It's completely improper.
He's specifically misstating the evidence.
MR. P. BAKER: Your Honor's arguing. You can answer.
If Colin said something different, I would have to take into account what he said in forming my opinion.
Okay. You can get strike that. Contamination the difference between contamination and cross-hybridization is difficult to ascertain, right?
And if a testing kit is prepared by Kirk and Zelmer (phonetic) is properly used you won't get the weak signals, correct?
If a kit is properly prepared by Perkin Elmer, you would not get these signals you're saying.
I'm not sure I follow your question exactly. There's experimental variations and even if one follows the protocol to the "T," it's possible that one can get cross-hybridization.
I may have seen it but again that is -- Is something that I never studied and looked at in any great detail.
Okay. I want to talk to you a little bit about some of the testimony you referred to a little bit earlier. (Exhibit 1279 displayed.)
It's an artifact or it's contamination. An artifact in a broad sense, it can be due to any number of experimental variabilities and if one is calling all those artifacts, it can be due to artifact or contamination.
Okay. And the positive control which is submitted in order to determine whether the DQ Alpha testing is being done properly didn't come back the way it was supposed to either, did it?
Let me rephrase it. That's not a very good question. The positive control was supposed to come back as a 1.1, 4?
That's correct I'm not sure that the 1.3 hint is actually what Colin Yamauchi would have written. I'm not sure he uses the same terminology but there is a very faint 1.3 that one can see.
And the 1.3 determination of LAPD item 30 should be questioned, but you had a 1.3 on the QC816 control and you had a hint on the CSC816 control and you had a hint of the 1.3 on the positive control, right?
Well, whenever one is looking and evaluating these strips, one has to evaluate what was done at LAPD and parallel with everything else that was done in that same run at LAPD, what was done at DOJ with everything else that was done with DOJ on that particular run.
Then when they looked at QC816, and the positive control, they discarded the 1.3 findings on the two controls, right?
Well if the control showed a 1.3 at the QC816 and it showed a 1.3 as a positive control, this 1.3 on LAPD item number 30 should be questioned as well. You'd agree with that, wouldn't you?
Okay. But they, nonetheless, determined that it was a 1.3 dot on LAPD item 30 regardless of the 1.3 dots on the other two controls, true?
Now, you've seen the other board which is civil 1281. Okay. Now civil 1 -- or civil 1281, get them mixed up with the numbers. Now, on this one, the positive control should have come back as a 1.1, 4, right?
Now, LAPD item number 52, that's a swatch collected on the Bundy walkway. You know that, right?
Now, on that one, in terms of item 52, they decided to ignore the 1.3 hint on the evidentiary item, right?
I don't want his explanation, they can argue it. They've heard it -- they've determined it to be a 1.3 hint but they ignored it, right based on the controls?
But they didn't put that down in they're results as the findings of the possible source of this blood on item 512, correct?
A.. Well on every sheet that I've seen in every board it's listed up there it's a 1.1, 1.2 and a 1.3? Mr. Simpson doesn't have a 1.3 allele.
Now, I want to show you the next board, Civil 1276. Now, you reviewed all of Dr. Gerdes's testimony, right?
And you had no opinions rendered on direct about his testimony as to the contamination in the reference samples, did you?
Okay. Now -- and this board also shows that OJ Simpson doesn't have a 1.3 allele on DQ Alpha line, right?
Well, it's one of the markers that is incorporated into the polymarker. And it stands for group specific compliment and it's just one of the genes that is incorporated into that test.
Okay. Now, in terms of the reference sample of Nicole Brown Simpson, the reference is sample supposed to be absolutely clean, correct?
They were examined, the reference sample on June 15 in the order of OJ Simpson, Nicole Brown Simpson and Ron Goldman.
Okay. Now the reference sample of Nicole Brown Simpson had a 1.1, 1.1, but also showed a 1.2, right?
Reference sample of Ron Goldman showed a 1.3, 4, and a 1.1 which is consistent with OJ Simpson, right?
No. It comes down to possibility of something versus the probability and what is the bottom line here is that one's dealing with samples that are very rich in DNA and Nicole Brown Simpson -- that's the way this test is designed. Any time you overwhelm the system with too much DNA, one can get cross-hybridization. When one would expect cross-hybridization is in samples that contain a reasonable amount of DNA, and so the explanation that was rendered by Dr. Cotton --
The explanation rendered by Dr. Cotton as to that faint allele, I would agree fully that the more likely explanation other than contamination is if, in fact, that is cross-hybridization.
And you are just trying to offer up your testimony as to why there is contamination or hybridization of the samples between the reference samples, right?
I wasn't asked to give an explanation. I was just asked if the type was consistent with Mr. Simpson.
It's the only one of the markers that's used in this set -- This study that we do not use, that's correct.
Okay. Now, in terms of the Bundy drops, you are aware, are you not -- we can take that down (indicating to exhibit). You are aware, are you not, Ms. Mazzola testified that she initialed the bindles, correct?
Objection. We went through this yesterday. It misstates the testimony.
MR. P. BAKER: Your Honor the objection was overruled.
What's the question?
MR. P. BAKER: You are aware that Ms. Mazzola has testified under penalty of perjury that she initialed the bindles?
Objection. Beyond the scope; has nothing to do with contamination.
MR. P. BAKER: We're talking about DNA Bundy swatching, the DNA testing of the Bundy swatches.
You may ask whether that was a factor he considered and ask him his opinion.
MR. P. BAKER: Was it a factor you considered that there was testimony that the swatches were dry when they were placed in the bindles?
Well, there was a lot of -- again, that was outside what I was asked to fundamentally do in this case. Therefore, there was a conflicting testimony about if they were dry, they weren't dry. So I don't know the answer to that. I mean my understanding is that maybe they were dry, maybe they weren't. I can't answer that. Again, that was not fundamentally important for me to know that to evaluate this evidence.
So the handling of the samples was not fundamentally important to your opinion as to whether there was contamination or not. Is that what you're saying?
Well, did you rely on some of the testimony that said that the bindles, that the swatches were wet when they were -- that the swatches were dry when they were placed in the bindles?
I am aware that there was testimony that there was a bit of transfer from some of the swatches onto the bindle, yes.
Now, in terms of the Bundy drops, you talked about a main factor in your determination was the cleanliness of the substrate controls, right?
Well, a substrate control is just simply a -- a sample that is removed from an area adjacent to where the actual stain is being picked up. And it's to tell you a couple things. It's to tell you if there's something that's present on that substrate, on that surface, could inhibit you getting results. And number 2 it's to tell you if there's anything else that's there, that you can't see, that's influencing the results.
So a substrate is almost, in a sense, synonymous with a surface that -- a substrate control of a carpet, you'll take it of a sidewalk, you'll take it of a driveway. The way forensic scientists use the term, it's synonymous with the surface it was taken from. The substrate controls in terms of bindle swatches were totally clean?
What do you mean immaculate?
MR. P. BAKER: There wasn't any dirt on any of them, was there?
I was -- again this was not fundamentally important to the DNA evidence in the case, but I thought there was testimony that some of those were pinkish in color.
There was no dirt on any of the Bundy swatches, as far as you know, as you sit on that stand today, right?
Asked and answered, Your Honor. He just answered it.
MR. P. BAKER: Not that question.
Well, the color that was transferred on some of these substrate controls is, in fact, evidence that whatever was there was picked up from that surface.
You think you may get some dirt if you took some substrate controls of a muddy, leafy walkway?
No foundation for any of this, Your Honor. It's also argumentative, and has nothing to do with his testimony. It's way beyond the scope.
MR. P. BAKER: I didn't hear the ruling.
Well, I've never looked at those photographs in any detail. I've seen copies of them, but I've never seen the actual exhibits. No, I have not.
(BY MR. P. BAKER) By the way, in terms of the reference samples, was there any contamination of OJ Simpson's reference sample?
(Continuing.) I'll tell you, as far as I know, there was no contamination or cross-hybridization of Mr. Simpson's reference sample, right.
Just for the record, we have the -- which defense exhibit is that?
MR. P. BAKER: You're looking at board 1275.
Either one. There was no showing of contamination or cross-hybridization of Mr. Simpson's reference sample, right?
That's correct. I don't believe that -- I don't recall there being any cross-hybridization.
Okay. Now, in terms of the swatches or the socks, rather, what was Nicole Brown Simpson -- what was the nanogram level of Nicole Brown Simpson's stain, the stain we've identified as that of Nicole Brown Simpson?
Again, I don't have those numbers committed to memory, but there was a lot in that stain, yes.
You mean -- are you saying is it consistent that somebody could have taken blood from a reference vial and put it on that sock and ended up with 1300 nanograms.
Show me where that is. Approach the bench. (The following proceedings were held at the bench, with the reporter.)
MR. P. BAKER: I don't have that much longer. I've got about --
He's talking about one examination. EDTA is a planting issue. This witness has nothing to do with EDT
And of course, we've just put on a witness who said there was no EDTA on this sock. This guy didn't know anything about. EDTA --
MR. P. BAKER: He gave conclusionary testimony as to all the evidence. They said, is this evidence reliable.
Where is your document that there was EDTA?
MR. P. BAKER: Mr. Rieders said there was EDTA on the sock.
He said there was EDTA on the socks -- could be EDTA on the socks. My guy said it wasn't. But this guy doesn't know anything about it.
THE COURT: Overruled. (The following proceedings were held in open court, in the presence of the jury.)
(BY MR. P. BAKER) Dr. Popovich, you are aware, are you not, that EDTA was found in the sample taken from the socks? True?
Mr. Lambert didn't tell you that Frederic Rieders had testified that there was EDTA found in the sample taken from the socks?
You may -- you know, you can ask whether or not anybody told you there was EDTA on anything.
I mean, what I'm looking at is DNA types and saying if there's consistency or inconsistencies based on DNA typing, not on mass spectrometry and EDT
A.
It would impact your opinion when you tell this jury that the evidence is reliable as to the socks and the back-gate stain, right, Dr. Popovich?
Again, I'm not an expert in EDTA and the techniques used to analyze EDTA, so I can't comment on that.
MR. P. BAKER: Your Honor, I ask that the witness be instructed to answer my question.
Objection. Misstates the testimony.
MR. P. BAKER: Concordant -- I don't want to misstate you -- was the concordant -- there was also 30 and 303 of the one Bronco console.
You didn't say that to this jury, that the samples of 30 and 303 taken on the console were concordant?
That test could only be run with PCR technology, right? You couldn't do an RFLP test on 31 because it wasn't strong enough?
Okay. Were you shown the picture taken on August 10 of the console by George Carmaney that did not show blood on that console where 303 was collected?
(BY MR. P. BAKER) Dr. Popovich, if there was contamination in the LAPD lab, you'd admit that contamination would have passed to Cellmark and DOJ in terms of these swatches, correct?
If a swatch was contaminated at LAPD, it could be contaminated when it went to Cellmark, DOJ, correct?
If all the swatches that were in evidence for a particular item were contaminated, not -- it wasn't the same swatch that went to each of the labs. That's why I'm being very precise here.
I'm just saying, sir, if the swatch was contaminated at LAPD, it would have been contaminated thereafter; true?
You have no independent knowledge, as you sit here today, whether or not there was contamination as to these evidentiary samples, independent knowledge, sir?
It's my -- my professional opinion that it -- it is not due to contamination. That's what I testified to.
There's always a possibility of contamination. But I believe it's not probable that there was contamination.
KEY QUOTEAll right. Have lunch, ladies and gentlemen. Come back at 1:30. (At 12:01 P.M., a luncheon recess was taken until 1:30 P.M. of the same day.) SANTA MONICA, CALIFORNIA; THURSDAY, JANUARY 16, 1997 1:30 PM DEPARTMENT NO. WEQ HON. HIROSHI FUJISAKI, JUDGE. APPEARANCES: (Per Cover Page) (REGINA D. CHAVEZ, OFFICIAL REPORTER) REDIRECT EXAMINATION BY MR. LAMBERT: (continued)
Every lab that does PCR has had contamination.
There's always a possibility of contamination. But I believe it's not probable that there was contamination.
I see absolutely no evidence of reagent contamination at LAPD. That is my testimony.
I wouldn't use the term 'hunky dory.'
Mr. Lambert and I never discussed anything about EDTA.