Thank you, Your Honor.
Mr. Yamauchi, can you give me an estimate of the total amount of time that you have spent working with or for the plaintiffs in this case?
You know, the time that you spent with the plaintiffs, how much time have you spent with Mr. Lambert?
Well, he didn't go over, like, specifically what he's going to ask, but he asked me questions.
How much experience did you have with case work in the PCR process at the time that you did the tests in this case?
How much experience did you have in case work with PCR analysis at the time that you did the tests in this case?
And there were other analysts at LAPD that had more experience than you, correct, in that topic?
Well, specifically in PCR, but in conventional and DNA serology, I was the most experienced.
(BY MR. BLASIER) Mr. Yamauchi, do you think it's important to have procedures in place to avoid the manipulation of samples, to avoid transferring material from one piece of evidence to another?
And that applies to evidence samples before they get to you, as well as when you get them; is that correct?
Anytime that a sample is looked at by you -- for instance, you have to unpack it from whatever container it's in, from the person who had it before?
It's very important each time you do that, that is, take it out of something and put it back, that you avoid too much manipulation of the item to avoid possible contamination, correct?
Now, in this case, you were aware right from the beginning that this was a very high profile case, were you not?
I didn't know it was going to be as high profile as it turned out to be, but we've had -- we work in LA. We've got a lot of high profile cases.
At the time of my testing, like I said, I knew it was a high profile case.
I didn't know it was going to be as big as it turned out to be.
Priority? Well, I was told that the results and things like that, there was reasons why they would need them rather quickly, yes.
Well, if I said and agreed to that, then I must have done it. I don't have a recollection of those specifics.
Now, the manual for the DQ Alpha system suggests that you not do more than 15 samples at a time; isn't that correct?
And for the 23 items you did on the 14th, that includes the preparation of the samples, the extraction of the DNA, the amplification of the DQ Alpha, the hybridization -- in other words, putting the solution on the testing strips, preserving the testing strips, and photographing the testing strips, correct?
Now, in your lab, you have to go to two different locations to do DQ Alpha tests, do you not?
And you have to start preparing the sample at Pipertech, which is where your lab is located, correct?
And then when you're ready to amplify a sample, that is to make multiple copies of small amounts of DNA, you've got to go to Parker Center, correct?
And after you do that, the amplified DNA, that has a lot more DNA than when you started, and you go back to Piper Tech, correct?
Well, no. Actually, we do the hybridizations over there. The only reason we take the DNA back, the DNA product back, is to do our product gels, which is done in a separate room from where the sampling takes place.
Okay.
But you bring back the amplified product from your amplification room and you bring it back to the lab; isn't that correct?
You do not have a procedure in place that prohibits taking amplified product out of the amplification area back to your lab; isn't that correct?
Unless I take the amplified product back to the lab, I can't analyze it on the product gel.
(BY MR. BLASIER) Okay.
Now, you can't do -- you can do what is called a yield gel, can you not?
(BY MR. BLASIER) Did you ever do a yield gel or a blot test to determine how much DNA you had and how much of it was human?
(BY MR. BLASIER) Have you been taught that cross-contamination is much more of a potential problem when you're dealing with smaller amounts of degraded DNA?
Contamination's going to be a problem, regardless of whether you have a large sample that contains a lot of DNA or a small sample in quantity of DNA.
Part of the problem is, though, you can't tell by looking at a blood swatch whether you have one blood swatch or a thousand blood swatches, what quantities or quality of DNA is in that swatch. So your point -- I'm not sure I really get it.
Okay.
Weren't you trained that where you have small quantities of degraded DNA, that cross-contamination, a little bit of it, can cause you to get improper results or incorrect results, because the small amount of contamination and the small amount of starting sample all amplified together?
Haven't you been taught that?
Small amount -- small amount of sample and small amount of contamination all amplified together.
Um-hum.
If you start with a larger quantity of DNA in your evidence sample, then a small amount of contamination is not going to be -- is not going to overwhelm your original larger amount, correct?
Well, the only thing we could use would be a blot. A gel is not effective with the type of extraction process that we utilize.
Excuse me. Okay.
From here on in, I will sustain the objection based upon the motion-in-limine ruling I made previously with regards to different collections in the different testing techniques.
And of testing techniques.
You may examine as to what he did, not whether there was any contamination as to what he did. We will not go into what other techniques there are available.
(BY MR. BLASIER) Mr. Yamauchi, before you were allowed to do case work, you were required to do proficiency testing, correct, and validation studies in your lab?
And you took a certain number of tests to determine whether you were capable of accurately reading DQ Alpha results, correct?
Do you recall the test that involved nine different vaginal samples that had combinations of vaginal fluids and semen?
You stipulated to the results. The question is contamination. You may examine as to his procedures re contamination.
BY MR. BLASIER: Now, Mr. Yamauchi, how did you get Mr. Simpson's reference blood on the 14th?
And when you started to take your notes about that reference sample, you put down that it was item 18, didn't you?
(BY MR. LEONARD) Mr. Fung didn't tell you when he gave you that sample, that it was really item 17, did he?
Now, that envelope was not sealed -- the envelope that had the vial was not sealed, was it?
And isn't it accurate that the procedure for those blood vials requires that that envelope be sealed at the time the blood is drawn?
(BY MR. BLASIER) Mr. Yamauchi, do you recognize this as a copy of the printing that appears on the front of the blood vial envelope?
I can't tell you that, since I'm not involved with that section of the collection process.
I really don't think I've ever read that.
It's supposed to be sealed from the time of collection until somebody does something with it; isn't that correct?
So it's left open -- evidence items are left open from the time they're collected until they're analyzed?
Now, when you got Mr. Simpson's reference vial, your testimony is that you took three chemwipes and put it over the cap of the vial?
And when you opened the vial, you got blood -- blood soaked through all three chemwipes, on your glove; isn't that correct?
Now, after you -- or, you said you took the cap off and you set it down on the table, correct?
And that was the cap that had all the blood that got through the three chemwipes, onto your glove, correct?
And you said unfortunately, you were in the evidence processing room.
You're not supposed to process those kinds of samples in the evidence processing room, are you?
Well, because it wasn't a familiar environment to me. If I were doing it back in serology, I would know where all my disposal receptacles are.
You have any idea how much blood got out of that vial when you took the cap off and put the cap down on the table?
Do you know how much of it got out on the chemwipe, on your glove, and the cap on the table?
You said on the table.
No blood got on the table, but soaking into the chemwipe and onto my glove, if I had to approximate, I'd say it's probably 10 to 20 microliters.
Three to five microliters of blood, well, if you were to put it onto a piece of paper or something like that, it would be a visible stain.
Not very big.
Maybe -- you know, this is a generalization -- it's going to depend upon the type of paper, of course, and other factors, but I would say approximately three millimeters in diameter.
Now, after the blood got out onto your glove and into the chemwipe, you took your gloves off and did something with it, correct?
Okay. After I replaced the cap, I had my gloves with the chemwipes in my hand, took my gloves off, and I realized I had to throw them out.
Once again, I had to decide whether to go to the back of this room, where they had the proper disposal receptacle, or to take them back to serology.
By the way, prior to the time you testified in the criminal trial, did you have any recollection at all of changing your gloves after you opened that cap?
My question was, before you testified in the criminal trial, do you -- did you have any recollection of changing your gloves?
And isn't it accurate that what happened at the criminal trial, after you admitted that you had opened the cap and got blood on the chemwipes and on your hands, that's when you remembered, I changed my gloves, too?
(BY MR. BLASIER) My question was, isn't it true that it was only after you admitted that blood had gotten out of the vial onto the chemwipes onto the glove, that you then remembered that you changed your gloves, also?
Now, after -- the first thing you did after you opened Mr. Simpson's reference vial and started processing that, was process the Rockingham glove, correct?
(BY MR. BLASIER) But while we're getting that, when you got the next day -- when you got the reference samples for both victims, those were personally delivered to you by Detective Vannatter, correct?
Okay.
And you didn't make any note of the quantity in those vials; of either of those, did you?
You would have no way of knowing whether there was less blood in those vials than had been put there by the coroner?
And you actually drew a diagram in your notes about how many times you handled or took things from the Rockingham glove, correct?
And each one of these arrows represents your taking something away or handling the Rockingham glove, correct?
And there are 1, 2, 3, 4, 5, times that you checked in the wrist area with phenolthalein testing or other samples, correct?
You did two phenol tests, three phenol tests, a spot-check, a sample cutting, did you not, in the wrist area of the glove?
Because that would be good procedure, wouldn't it?
You don't have anything in your notes that tells you what you did in processing these samples with respect to changing your gloves by any law enforcement officer, do you?
Specific recollection, no.
Isn't it true that it was only after you admitted that blood had gotten out of the vial onto the chemwipes onto the glove, that you then remembered that you changed your gloves, also?
Yes, because --
No, you don't; you need to answer the question.
Well, I said 'unfortunately' because it wasn't a familiar environment to me. If I were doing it back in serology, I would know where all my disposal receptacles are.