(BY MR. LAMBERT) And, in fact, Dr. Blake is one of the leading developers of the PCR techniques?
Now, Mr. Sims, Dr. Cotton has previously put on this board some frequency results based upon her DQ Alpha and polymarker test.
You see her frequencies up there?
Now, the Department of Justice also did a DQ Alpha test on these particular samples, didn't it?
In addition, however, the Department of Justice did a D1S80 test which Ms. Montgomery just testified to, and the results of that test are up on the board there; see that?
Now, taking that additional test into account, that is, taking it into account, the D1S80 test in addition to the DQ Alpha test and a polymarker test, what would be the frequency of all three of those tests combined?
Steve, you can help by erasing here, right there. And we'll ask you, Mr. Sims, to put the frequency in there.
(BY MR. LAMBERT) So, Mr. Sims, taking these three test results, the two from Cellmark and the one from the Department of Justice all into account, you would expect to find those DNA patterns in only one of 240,000 to one in 2.2 million people; is that right?
Your Honor, I'm going to object to erasing data from this exhibit which is now in evidence. He can put it in beside it, but I think he just erased something from the top.
I haven't moved this in; I'm developing this exhibit as I'm going along. I'll move it in when it's done.
Excuse me. If you want to preserve it, why don't you go up there and make an overlay and write it down on your overlay? We'll give you that opportunity right now.
Well, you can -- I've used an overlay before. I would like to not deprive you of the opportunity of showing the exhibit as it existed prior to that time that this witness changed it. So if you want to do that, you may.
(BY MR. LAMBERT) Going now to item number 48, again, this is an item where the Department of Justice did a DQ Alpha test?
And again, you did a D1S80 test which Ms. Montgomery already testified to.
Can you put in the frequency, taking that test into account?
(BY MR. LAMBERT) Okay.
Now, on item number 49, that's not an item that the Department of Justice did any tests on?
Let's take a look at item number 50.
It appears that the results you got there are the same as 47 and 48; is that right?
(BY MR. LAMBERT) And now, finally, on item number 42, that is finally -- 52, finally, on the Bundy list of items again, you have both DQ Alpha and D1S80 results on that item?
And then, now dropping down to item No. 115, which is from the rear gate of Bundy, you did your own DQ Alpha test there; is that right?
And the D1S80 Ms. Montgomery testified was 24, 25, those results, the DQ Alpha results and the D1S80 results together, who could be the possible source of those test results from among Mr. Simpson, Nicole, and Ronald Goldman?
Now, the reason that the numbers for item number 115 are different than, say, item number 52, is that explained by the fact that the more genetic markers that you're able to test, the more the frequency can become less common?
(BY MR. LAMBERT) What's the explanation of the difference between the 115 and, say, number 48?
Basically, the more genetic information you're getting, the more types you're looking at, then the rarer that whole profile becomes.
Now, finally, on this board we have item number 117 the rear gate.
From the rear gate, I see you have both a DQ Alpha and RFLP results; is that right, sir?
That's correct. We did DQ Alpha, D1S80 and we also did a nine-probe match for the rear-gate sample.
When doing RFLP, we looked at nine different loci. In other words, we produced nine different Autorads at different sides of genetic information take's all nine matched Mr. Simpson's types.
That last item, the item from the rear gate, because you got a nine-probe match on that item, the frequency, would you call that a rare frequency?
And the fact that you were able to get an RFLP result off of that particular evidence item, why could you get it off that, why not off of 115 or 116?
There was less DNA recovered in those stains, 115 and 116. 117 had about a little over 100 nanograms, which is sufficient for an RFLP.
And was that particular evidence item degraded or undegraded?
Do you recall what the state of degradation was?
And is there a reason why an evidence sample that's found in one location, say on a gate as opposed to another location, say on the ground, may be more or less degraded?
Yes. It's certainly important how -- what the micro environment is where a stain occurs.
For example, if it occurs on a walkway, where there's a lot of soil and that sort of thing, one may expect to see some differences from a sample that's not in contact with as much dirt and soil, debris, that sort of thing. The collection and processing can make a difference too.
So the fact that there's more DNA on an evidence item found up on a gate, as opposed to something on the ground, is that a surprising result, from your perspective?
(BY MR. LAMBERT) Now, this board lists four different evidence items from the -- collected at Rockingham. The first one, number 6, is from the Rockingham driveway.
Did you do any DQ Alpha tests on that evidence item?
Who would be the possible source among the three we're looking at here, be a possible source for those evidence items?
Okay. I think that's the only one we have on this, chart. So let's move to the next one, which is number 293.
(BY MR. LAMBERT) Now, there's a lot of evidence items to go through on this.
The Department of Justice tested a lot of samples from the Bronco automobile?
And for these items here, where we just have D1S80 results 25 and 26, numbers 25 and 26, are those both consistent with Mr. Simpson as a possible source, as well?
For the number 25, that range would be -- check for a second.
That's where we have a type 1.1, 1.2, Also a 24, 25 result, so the range would be 1 in 520 to 1 in 1,400. Where we have just the D1S80 result of type 24, 25, that would be one in 29, to one in 48.
And then while we're here, let's take a look at item number 30 from the center console.
Did you do a DQ Alpha test on that item?
Tallying the DNA and D1S80 results for that type, who is a possible source for that particular item?
Again, this is where we have the DQ Alpha and the D1S80, it would be one in 520 to one in 1400 or 1,400.
That -- excuse me. The sample No. 31, which is our 18, we obtained a mixture result. The DQ Alpha indicated the type 1.1, 1.2, along with the weaker typing of 1.3, comma, 4.
And then let's drop down to -- now to number 34.
The Department of Justice do a DQ Alpha test on that item?
Yes; that was our item number 20. And we did just the DQ Alpha test on that.
The result was 1.1, 1.2.
Let's drop down to item number 303, the center console.
Did you do a DQ Alpha test for that evidence item?
Yes. This was our item number 52.
We obtained a DQ Alpha result with 1.1 was present, possible 1.2 allele a 1.3 was present, and also a 4 was present on DQ Alpha.
Because of the D1S80, it's always 24, 25, with a weaker 18.
All three principals were potential contributors to that blood.
Yes. That was DQ alpha type 1.1, with a possible 1.2. 1.3 was there, also 4. The D1S80 was 24, 25, with a weaker 18.
And then evidence item number 305 from the center console, what were your results on that test?
In that case, it was 1.1, possible 1.2, then a weaker 1.3 and 4.
And then on the D1S80, it was 24, 25 and weaker 18.
These items, 303, 304, and 305, all tests from the center console, were those all mixtures of more than one person's blood?
Yes. What I was able to do, because individually these samples had relatively low amounts of DNA, but they did have enough combined to make an RFLP typing feasible, I proceeded to combine all of them.
In other words, the extracted DNA, I combined all of that, did an RFLP analysis on that, and then probed it.
We have four-probe RFLP analysis on that sample.
The four-probe RFLP match is consistent with the mixture of DNA from Mr. Simpson and also from Ronald Goldman.
And one sees a stronger contribution that lines up with Mr. Simpson's bands and a weaker contribution that lines up with Mr. Goldman's bands.
KEY QUOTEThank you.
Why don't you have a seat.
Before we leave the Bronco automobile, let me ask you about one of the other items there; that is item number 29.
Did you do a DQ alpha test on item number 29?
In item number 29, which I believe is a steering wheel, we got a type -- main type of 1.1, 1.2, which was consistent with Mr. Simpson, and we also detected a weaker 4 allele by itself.
And did you make any conclusion as to who among these three could possibly be excluded, based upon that test?
Well, as far as the exclusion, certainly the 1.1, 1.2 is the major type, is consistent with Mr. Simpson.
The 4 allele was identified as being present.
The 1.3 of Mr. Goldman was not seen in that particular sample.
My opinion was that this appears to be an exclusion of Mr. Goldman, but I could not be absolutely certain because of some of the technicalities of this particular case that we may not -- we, at the very low level of DNA, we were looking that, potentially, we would lose one of Mr. Goldman's alleles or one of his dots, basically, but I should point out there was no evidence of the 1.3.
(BY MR. LAMBERT) This board lists results of tests by the Department of Justice on the Rockingham glove. Renee Montgomery already testified to some of these test results.
Mr. Sims, it appears that a significant number of tests with were run on the glove; is that true?
Let's just then go through them, if we can.
On item number -- what you call this is your nomenclature, G1?
On that particular test, it indicated a mixture with a type 1.1, 1.3, a 4, and then also a possible 1.2 allele.
Yes. I looked at an RFLP on three of those; we did an RFLP; then a fourth one, we did an RFLP by itself.
And the possible sources of people, pursuant to your tests for this particular item, are who, sir?
And in your five-probe RFLP test results, were you able to see the bands matching both Nicole Brown and Ronald Goldman?
Yes. It was consistent with a cross -- with a mixture of DNA from Nicole Brown and Ronald Goldman on that sample.
So for that particular sample, then, you have both a five-probe RFLP test, a DQ alpha test, and a D1S80 test that are all consistent with the blood coming from Nicole Brown and Ronald Goldman?
Yes. Again, we saw that same type of person, indicating a mixture consistent with Nicole Brown and Ronald Goldman.
Now, let's go to -- we've already done this one.
Let's go down to area G4.
And can you tell us what your results were there, sir?
And were you able to, at all five of those genetic markers that you probed, able to see the banding appearance for both Ronald Goldman and Nicole Brown?
Well, what was interesting especially, was that we looked at G1, G2 and G4, across one auto series of Autorads, and it was apparent that the bands that could be attributed to Ronald Goldman were fairly constant in intensity across the Autorads, whereas the bands that lined up with Nicole Brown's bands tended to fade out, going from G1, being the stronger, a little weaker for her at G2, and then weak at G4.
Well, I think given that you could see the relative contributions, it was highly significant that this was a very clear -- clearly consistent with the mixture of the two.
In other words, you could figure out which band could go to which individual.
Again, that was a mixture of possible -- possibly a mixture. I saw on DQ Alpha we had a type 1.3 and 4 present. There was a possibility of a 1.2 allele and a possible trace of a 1.1 allele.
And the possible contributors to this evidence item, then, that you were able to ascertain, would be who?
Well, looking at that result with the D1S80, as well as the DQ alpha, we could only say that it -- with some confidence, that it was consistent with Ronald Goldman.
Even though you had a possible 1.2 in this, you wouldn't conclude that there's another possible contributor among the three?
Yes. For example, with the possible trace 1.1, we would need a clear 1.1 and some more information on the D1S80 to attribute it to either Nicole Brown or Mr. Simpson.
On that one, again, it was a 1.3, comma 4, possible 1.2, and then a weaker 1.1 allele was detected.
(BY MR. LAMBERT) Now, Mr. Sims, did you receive a pair of socks that were found in Mr. Simpson's master bedroom floor and marked Exhibit 13?
At that point in time, LAPD had detected and analyzed some of the blood stains present on them, and we were asked to do DNA typing on those blood stains.
In the initial examination of the socks, in a large -- relatively large stain had been cut out around the ankle area of one of the socks.
There was also on that same sock, a -- on the opposite side of that stain, there was an area indicated with a white arrow. Then there was also on the other sock, there was an area that had been outlined previous to my examination.
Yes. I examined the socks. First I photographed them to document what was present. And in the process of photographing those socks, and under very bright camera lights, photography lights, I noticed that there were additional stains on those socks.
At that point, then I later did some stereomicroscopic examination of the socks and determined there were a lot of very small blood stains on those socks.
And were those blood stains that you had not seen with your naked eye before you did the stereomicroscopic examination?
Yes, he was there at the time we initially examined the socks, and he was there for some of the later examinations.
Let's start with the one up at the top here, which is your number 42A-1.
Did you do a DQ alpha test on that one?
Yes.
And I should point out, that is the large stain (indicating), and LAPD had previously cut out some of that stain and submitted that to me in a tube, along with the socks themselves.
Can you please put in the frequency numbers for that 11 probe match on that particular evidence item.
Yes.
The RFLP loci this is just 6 of those 11 probes the numbers come out to be 1 in 7.7 billion, with a B, to 1 in 41 billion.
KEY QUOTEAnd did you do a calculation for the frequency of those two types, counting the D1S80, as well?
And we have on the board here a nine-probe match.
Is that consistent with your recollection?
Can you put the frequencies up on the board for the nine-probe match on that evidence item.
Again, this is just for six of those nine.
The numbers come out to be one in 57 billion to one in 150 billion.
And the D1S80, we already heard from Renee Montgomery.
Who would the possible source of that be?
Now, we're down to number 42B-1.
And would you tell us what the DQ alpha results were there.
The DQ alpha result on that case was a type 1.1, 1.1.
We also thought there might be a possible trace of a 1.2 allele.
And the possible trace of 1.2, were you able to include anyone as a possible source based upon that?
Well given -- given that I couldn't really say it was there, I wouldn't make anything on that -- out of that result, that possible trace.
And then finally, on this chart, we have item -- your item 42B-2.
What are your results on that, sir?
On 42B-2 was the same, 1.1, 1.1 DQ alpha, possible trace of the 1.2, and the D1S80 was 18, 18.
Because there was only a possible trace, you wouldn't include that in your characteristics of the possible sources for that item?
So did you put frequency in for that item, just 1.1 and the 1.1 and the 18, 18 D1S80 results?
The range on the RFLP was 1 in 57 billion, with a B, to one in 150 billion.
Yes; this would be a rare event.
The four-probe RFLP match is consistent with the mixture of DNA from Mr. Simpson and also from Ronald Goldman. And one sees a stronger contribution that lines up with Mr. Simpson's bands and a weaker contribution that lines up with Mr. Goldman's bands.
On that particular sample, it would be Nicole Brown and Ronald Goldman.
The RFLP loci this is just 6 of those 11 probes the numbers come out to be 1 in 7.7 billion, with a B, to 1 in 41 billion.