Dr. Cotton, can you give us an estimate of the amount of time that you spent on this case when you were working for the prosecution, just approximately?
And the tests that were done on the 23 items were paid for by tax payers of Los Angeles County, correct?
And all of your time that you spent in the criminal case were paid for by the taxpayers of Los Angeles County?
Now, it's -- have the plaintiffs reimburseed taxpayers of Los Angeles County for any of that work.
For my time here, the company will charge $1200 a day for time that I spend in the office. Some of that time is in short chunks and doesn't really get charged at all and if it's a substantial block of time, it would be $200 an hour.
You have no personal knowledge as to how the evidence was collected, preserved and packaged before it was sent to your lab?
Would you agree with your -- that your test results are only as good as the evidence that you're given by an outside agency?
And if you're given evidence from an outside agency that has been contaminateed or degraded or tampered with or whatever, you can't make it any better, can you?
KEY QUOTENow, I want to ask you a couple more questions about what DNA is to try and give us a conceptual framework of what we're talking about. Every cell that has a nucleus has DNA in it, correct?
So if you take some DNA from hair, it's going to be the same as DNA from your blood from your skin, from other bodily tissue, correct?
And that's one of the ways you can compare hair sample with blood sample with DNA technology, correct?
Now, going back down to the lower level here, the structure of DNA, you described it as a ladder type structure?
Now, in a single cell the DNA in one cell is you have 6 billion of these base pairs or 6 billion rungs of the ladder in every cell, correct?
We can think of it in terms of a tinker toy ladder that has two hubs and a stick in the middle through the rung. And the left hub is going to be an A and the right hub is going to be a T. The right hub could be a C and the left hub could be a G?
You can figure out how these go together, round letters go together. A and C and G -- I'm sorry, guess you can't do that. Anyway, they only go together one way, correct?
Okay. Now, 3 billion base pairs, if we want to try and understand the scale of this, if you think about a little toy ladder, like a kid might have on a fire engine, if the rungs of that ladder are half an inch apart, the entire DNA, if it was stretched, end to end, would be 3 billion half inches, correct?
Once around the world.
And the second 3 billion pairs of rungs, that comes from the other parents, is the same length, correct?
Okay.
So you can think of this as two ladders that are side by side that go around the world?
Now, when you do an RFLP test, would you say, is the -- let me ask you this:
Isn't it true that if you have the DNA from a piece of evidence and the DNA from a suspect, if there is a single rung of that ladder that is different from one to the other, it came from different people?
Thank you.
And when you do an RFLP test, what is the average fragment lengths that you look at?
Your -- the range of fragment length that are looked at on our gels are 1600 to about 23,000 base pairs.
Okay. So from 1600, which is about 800 inches, under my scenario, to 23,000 half inches or about 12,000 inches, correct?
That's all you look at?
And the polymarker system that -- the alleles that you look at in the polymarker system are even smaller, aren't they?
They're in the neighborhood of 250 base pairs, correct?
I don't know about your numbers. If we just stick with base pairs, then I'll be able to answer your questions a little bit better.
Now, you actually don't have one continuous piece of DNA that goes all the way around the world. It's broken down into 23 sections?
So it's, you know, one twenty-third, approximately, of the ladder is going to be one chromosome and scientists have given these numbers, chromosome number 1, number 2, et cetera?
And if you go down below, within a chromosome, they're broken down into the smaller segment or they're blocked out in smaller segments that are called genes, correct?
Okay. And a gene is simply a series of base pairs that could have a wide variations in length, but it's just by definition, scientists have defined certain things to be a gene?
So a particular section of the ladder that might have 100,000, 200,000 base pairs scientists might say, we think that this is connected to hair color, for instance, and so that's a gene that might be conected to hair color?
An allele could be very long. It could be very short. It doesn't -- there's no connection between an allele and length.
Well that's correct for this test. But you could have an allele of a gene that was 100,000 base pairs.
Okay. If the roughly 10,000 base pairs average from 1600 to 23, -- 20,000 -- excuse me -- and in it's 5 probe matches that you were talking about, there are two bands for each probe, correct?
And again, we're talking about two side by side segments of the ladder; one from mom, one from dad in each probe?
And those five segments total together, if our average is 10,000 base pairs per segment, is only 50,000 base pairs that you're looking at for an RFLP test?
I'm not sure how to -- The way to answer that question is to think of a drop in a more precise volume. We get about 10,000 nanograms from an amount of blood that would be a little less than the size of this pen top.
You heard the figure that there are 1,000 to 2,000 nanograms per -- I'm sorry. Give me that again, your figure of how many nanograms in a -- in a size of blood the size of --
We get about 10 micrograms, which is about 10,000 nanograms in about 700 microliters of blood.
So you could calculate, sort of. You could go down and workout how many -- what volume of blood would produce a certain amount of nanograms and I haven't done that recently.
Okay. Just to get a rough estimate, 20 nanograms of blood is going to be extremely small; is it not?
I don't know. I mean, how big a drop is, is a very subjective thing. So in the laboratory, you don't measure things in terms of drops. So I don't really have a figure for you -- for that. I don't know.
I haven't heard any physician -- I don't know a common figure for a drop. I'm not aware that there's a common figure for a drop.
There may be such an estimate. I'm just not aware of it, you know. That's just the state of what I know.
I'm going to try and draw this.
Now there are a number of different terms that are used in forensic science for when two things appear to be -- or could come from the same source, aren't there?
And none of the tests that you do are capable of establishing or do establish unique identity, do they?
Well, the estimate that you come up in terms of numbers are statistical estimate based on some formulas that you do, correct?
And you cannot possibly look at all of the DNA in a sample. You're only looking at a very small part of it, correct?
If you do, if you have available to you, a series of a large enough series of problems on an RFLP test. I'm not saying that five is necessarily large enough. I'm just saying if you have enough, I would say perhaps ten or more, I don't think there's a scientist who would argue with you that you've established identity.
But no scientist will tell you that with five probes you've established a unique identification, will they?
I don't think that. Yes, I agree that five probes would not be necessarily considered to be an identification.
And you've heard the term "match" used in the context of, for instance, hair and fiber evidence where it means a hair could have come from the suspect or, then again, it might not have, right?
(BY MR. BLASIER) I'm trying to name what a match is for purposes of the jury's understanding this. Okay, Doctor?
Now, with RFLP technology, which provides the most information, you look at bands on an X-ray, correct?
Okay. But -- and if we consider one band to be from the evidence and one band to be from the suspect -- you with me so far?
And with RFLP, you look at an average of 10,000 base pairs per band, an easy round number, okay?
So let's say that you have determined that the evidence band is 10,000 base pairs?
In order for your suspect to match the evidence, the suspect's corresponding band has to be 10,000 base pairs correct?
Well, let me rephrase it.
In order for the DNA from the suspect to be identical to the DNA from the evidence, it has have the same sequence and the same number of base pairs?
Okay. So for identity to be established, would this one section, anyway, you've got to have 10,000 base pairs from the suspect that matches exactly the 10,000 from the evidence, correct?
You have a lot of other DNA that you're not even looking at, you're only looking at one small piece?
Correct.
Now, if it turned out that your suspect band was 9,999 and your evidence band is 10,000, those two samples came from different people, correct?
We'll do this step by step. Okay?
Now, for 10,000 base pair segments, what is the window size that you use -- explain that in a minute. Explain to me what the window size is approximately in percentages?
Now, if you have a suspect sample, that is, percentages are 9,500 base pairs long and your evidence sample is 10,000, you know that these came from two different people if you know those numbers precisely?
Okay. And further you would know that if the suspect's base -- Fragment was 10,500 base pairs long you would know, if you were able to determine those numbers precisely, that these two samples came from different people, correct?
And virtually all there are a thousand possibilities between 10,500 and 9,500 of base pair lengths, correct?
And because the measurement techniques that are used are not sophisticated enough to measure fragment length you give yourself a window within which to call something a match, don't you?
What you say is gee, since we can't measure this very precisely we're going to say if it's 10,500 base pairs, we're going to call it a match to 10,000, aren't you 'cause that's within your window; isn't it?
Well, forgetting the math for a minute, if it's within the window, then it's called a match. And if it's not within the window, then it would either be an inconclusive or an exclusion.
Okay. So every one of the thousand possibilities in here within this range, you will call a match. But only one of them out of the thousand is truly a match, correct?
KEY QUOTETheoretically, if you were -- theoretically, yes, what you're saying is absolutely true.
KEY QUOTEIt's not a measure -- matter of measuring it good enough, it's what is the technique able to do.
The technique is unable to measure it with any, or more precision than this, correct, Doctor?
And the wider this window is, the more -- the greater the chance is that you're going to call somebody a match, a suspect and an evidence band as a match, when it came from different people, correct?
Oh, you don't. Okay. And this is true, this window that you use, because of measurement, is used for every one of the problems that you use in RFLP, correct?
So for a five probe match, where you're looking at ten bands, you've got this, plus or minus, roughly five percent. I know it changes for the size of the band, but for the sake of discussing it, you've got this same window, if you will, on every single one of those bands, don't you?
(BY MR. BLASIER) The one you gave was 530 billion. I think it was a five probe match using this technique. Would you allow yourself a large -- or a window -- I won't say a large window, to call something a match when you can't tell that it's the same, can you?
And your computer program that you talked about, that doesn't make it any more accurately than this either, does it?
Computer program, I talked about the computer imaging system; is that what you're referring to?
The computer imaging system is what you use to come up with the number in base pairs that the bands are.
Okay. Of your 1 in 530 billion sample that you told us about, which was -- which one was that?
If you're given evidence from an outside agency that has been contaminated or degraded or tampered with or whatever, you can't make it any better, can you? No.
Every one of the thousand possibilities in here within this range, you will call a match. But only one of them out of the thousand is truly a match, correct?
Theoretically, if you were -- theoretically, yes, what you're saying is absolutely true.
I don't think that five probes would be necessarily considered to be an identification.